| Species: | Human |
Expression Host: | Human 293HEK cells |
| Expression cDNA Clone and Sequence |
Recombinant protein was produced with TrueORF clone, RC226414. Click on the TrueORF clone link to view cDNA and protein sequences. |
| Tag: | C-terminal MYC/DDK |
Predicted MW: | 184.6 kDa |
| Purity: | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Mass Spec Validation: |
This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. |
| Concentration: | >50 ug/mL as determined by microplate BCA method |
| Buffer and Storage: | 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. |
| Preparation: |
Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| BioActivity: |
DNMT1 activity verified in a biochemical assay: DNMT1 (DNA (cytosine-5-)-methyltransferase 1) (TP326414) is a key methyltransferase that is responsible for maintaining methylation patterns established in development. DNMT1 preferentially methylates hemi-methylated CpG di-nucleotides and associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand. Varying concentrations of DNMT1 were added to a microplate containing a bound methyltransferase substrate. After incubation, the resulting methylated DNA residues were detected immunologically and a colorimetric signal was generated and measured. |
| Protein Families: |
Transcription FactorsDruggable Genome |
| Protein Pathways: |
Cysteine and methionine metabolismMetabolic pathways |
