||Expression Host:||Human 293HEK cells
|Expression cDNA Clone and Sequence
Recombinant protein was produced with TrueORF clone, RC223277. Click on the TrueORF clone link to view cDNA and protein sequences.|
||Predicted MW:||47.2 kDa|
|Purity:||> 80% as determined by SDS-PAGE and Coomassie blue staining|
|Concentration:||>50 ug/mL as determined by microplate BCA method|
|Buffer and Storage:||25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.|
||Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
||The specific activity of KATII was determined by measuring the product Kynurenic acid formation from a conversion of Kynurenine. The reaction was carried out at 37? for 15min in the buffer containing PBS, pH7.4, 2mM a-oxoglutarate, 40µM PLP (pyridoxal 5'-phosphate), and 0.5mM kynurenine as the substrate.
||Lysine biosynthesisLysine degradationTryptophan metabolismMetabolic pathways