RAPSN (NM_005055) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC222710. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 46.1 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Druggable Genome Protein Pathways:

Protein Data

RefSeq: NP_005046	RefSeq Size: 1664	RefSeq ORF: 1239
LocusID: 5913	Cytogenetic: 11p11.2
Synonyms : RAPSYN; RNF205
Summary: This gene encodes a member of a family of proteins that are receptor associated proteins of the synapse. The encoded protein contains a conserved cAMP-dependent protein kinase phosphorylation site, and plays a critical role in clustering and anchoring nicotinic acetylcholine receptors at synaptic sites by linking the receptors to the underlying postsynaptic cytoskeleton, possibly by direct association with actin or spectrin. Mutations in this gene may play a role in postsynaptic congenital myasthenic syndromes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Apr 2011].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens receptor-associated protein of the synapse (RAPSN), transcript variant 1 as transfection-ready DNA • Lenti ORF clone of Human receptor-associated protein of the synapse (RAPSN), transcript variant 1 , Myc-DDK-tagged • Lenti ORF clone of Human receptor-associated protein of the synapse (RAPSN), transcript variant 1, mGFP tagged • GFP-tagged ORF clone of Homo sapiens receptor-associated protein of the synapse (RAPSN), transcript variant 1 as transfection-ready DNA • Myc-DDK-tagged ORF clone of Homo sapiens receptor-associated protein of the synapse (RAPSN), transcript variant 1 as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of receptor-associated protein of the synapse (RAPSN), transcript variant 1 Validated Antibodies • Goat Anti-RAPSN / Rapsyn Antibody • RAPSYN Rabbit Polyclonal (C-Terminus) Antibody • RAPSYN Rabbit Polyclonal (C-Terminus) Antibody Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • RAPSN - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • RAPSN - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Rapsn - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • RAPSN - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Rapsn - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene RAPSN

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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