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Home Recombinant Human Proteins All ALK Proteins

ALK (NM_004304) Purified Human Protein

Specifications Citations Customer Service Product Documents
Cat. No. Description Datasheet Price Availability*  
TP322485 Recombinant protein of human anaplastic lymphoma receptor tyrosine kinase (ALK), 20 ug (Larger pack size?) $680 In Stock
TA800730 ALK mouse monoclonal antibody, clone OTI1E12 (formerly 1E12) $379 In Stock
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OriGene Data
Species:Human Expression Host:HEK293 cells
Expression cDNA Clone or AA Sequence
Recombinant protein was produced with TrueORF clone, RC222485. Click on the TrueORF clone link to view cDNA and protein sequences.
Tag:C-terminal MYC/DDK Predicted MW:176.3 kDa
Purity:> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration:>50 ug/mL as determined by microplate BCA method
Buffer:25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
BioActivity: ALK activity verified in a biochemical assay: ALK (anaplastic lymphoma receptor tyrosine kinase) (TP322485) activity was measured in a homogeneous time-resolved fluorescent (HTRF®) assay. ALK is an orphan receptor protein-tyrosine kinase having a putative transmembrane domain and an extracellular domain. Varying concentrations of ALK were added to a reaction mix containing ATP and a biotinylated kinase substrate and the reaction mixture was incubated to allow the protein to phosphorylate the substrate. HTRF detection reagents were then added, and the time-resolved fluorescent signal was measured on a Flexstation 3 microplate reader. The time resolved fluorescent signal is expressed as “delta R” or “ΔR” and is a ratio calculated from the fluorescent emission intensities of the donor and acceptor fluors.
Protein Families: Protein KinaseDruggable Genome
Protein Pathways:
Protein Data
Protein Image
BioActivity Data
BioActivity Image
 

Reference Data
RefSeq: NP_004295 RefSeq Size: 6222 RefSeq ORF: 4863
LocusID: 238 Cytogenetic: 2p23.2-p23.1  
Synonyms : CD246; NBLST3
Summary:
*: Inventory for certain proteins may be limited due to low expression level. Delivery time may vary from web posted schedule. Contact techsupport@origene.com for specific inventory information
**: DDK-tag is the same as FLAG tag. Flag® is a registered trademark of Sigma-Aldrich

 

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