NOS1 (NM_000620) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC222384. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 160.8 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Druggable Genome Protein Pathways: Arginine and proline metabolismMetabolic pathwaysCalcium signaling pathwayLong-term depressionAlzheimer's diseaseAmyotrophic lateral sclerosis (ALS)More Pathways >>

Protein Data

RefSeq: NP_000611	RefSeq Size: 7124	RefSeq ORF: 4305
LocusID: 4842	Cytogenetic: 12q24.2
Synonyms : bNOS; IHPS1; N-NOS; NC-NOS; nNOS; NOS
Summary: The protein encoded by this gene belongs to the family of nitric oxide synthases, which synthesize nitric oxide from L-arginine. Nitric oxide is a reactive free radical, which acts as a biologic mediator in several processes, including neurotransmission, and antimicrobial and antitumoral activities. In the brain and peripheral nervous system, nitric oxide displays many properties of a neurotransmitter, and has been implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. This protein is ubiquitously expressed, with high level of expression in skeletal muscle. Multiple transcript variants that differ in the 5' UTR have been described for this gene but the full-length nature of these transcripts is not known. Additionally, alternatively spliced transcript variants encoding different isoforms (some testis-specific) have been found for this gene.[provided by RefSeq, Feb 2011].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens nitric oxide synthase 1 (neuronal) (NOS1), transcript variant 1 as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens nitric oxide synthase 1 (neuronal) (NOS1), transcript variant 1 as transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of nitric oxide synthase 1 (neuronal) (NOS1) Validated Antibodies • Rabbit Monoclonal Antibody against NOS1 (Clone EP1855Y) • NNOS / NOS Rabbit Polyclonal (aa1422-1433) Antibody Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • NOS1 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • NOS1 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Nos1 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • NOS1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Nos1 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene NOS1

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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