ZAK (NM_016653) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC221970. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 91 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Protein KinaseDruggable Genome Protein Pathways: MAPK signaling pathwayTight junction

Protein Data

RefSeq: NP_057737	RefSeq Size: 3767	RefSeq ORF: 2403
LocusID: 51776	Cytogenetic: 2q24.2
Synonyms : AZK; MLK7; mlklak; MLT; MLTK; MRK; pk
Summary: This gene is a member of the MAPKKK family of signal transduction molecules and encodes a protein with an N-terminal kinase catalytic domain, followed by a leucine zipper motif and a sterile-alpha motif (SAM). This magnesium-binding protein forms homodimers and is located in the cytoplasm. The protein mediates gamma radiation signaling leading to cell cycle arrest and activity of this protein plays a role in cell cycle checkpoint regulation in cells. The protein also has pro-apoptotic activity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens sterile alpha motif and leucine zipper containing kinase AZK (ZAK), transcript variant 1 as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens sterile alpha motif and leucine zipper containing kinase AZK (ZAK), transcript variant 1 as transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of sterile alpha motif and leucine zipper containing kinase AZK (ZAK), transcript variant 1 • Transient overexpression lysate of sterile alpha motif and leucine zipper containing kinase AZK (ZAK), transcript variant 1 Validated Antibodies • Rabbit polyclonal ZAK (Ab-165) antibody • Rabbit polyclonal anti-MLTK (ZAK/AZK) antibody Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • ZAK - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • ZAK - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • B230120H23Rik - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • ZAK - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • B230120H23Rik - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene ZAK

If you would like OriGene Technologies to prepare a purified protein in the quantity of your own interest, simply fill out the form and click Submit. OriGene will contact you.

Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
* Quantity (µg)
Comments
* First Name
* Last Name
* Email Address
* Institution Name
* Country
* Phone