ZAR1 (NM_175619) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC221731. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 45.7 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Protein Pathways:

Protein Data

RefSeq: NP_783318	RefSeq Size: 1438	RefSeq ORF: 1275
LocusID: 326340	Cytogenetic: 4p11
Synonyms : MGC123986; MGC123987; zygote arrest 1
Summary: The female gamete, the oocyte, serves the distinct purpose of transmitting the maternal genome and other maternal factors critical for postovulation events. Oocytes have diverse functions in ovarian folliculogenesis, fertilization, and embryogenesis. ZAR1 is an oocyte-specific gene that appears to function at the oocyte-to-gamete transition (Wu et al., 2003 [PubMed 12539046]).[supplied by OMIM, Mar 2008].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens zygote arrest 1 (ZAR1) as transfection-ready DNA • Lenti ORF clone of Human zygote arrest 1 (ZAR1) , Myc-DDK-tagged • Lenti ORF clone of Human zygote arrest 1 (ZAR1), mGFP tagged • GFP-tagged ORF clone of Homo sapiens zygote arrest 1 (ZAR1) as transfection-ready DNA • Myc-DDK-tagged ORF clone of Homo sapiens zygote arrest 1 (ZAR1) as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of zygote arrest 1 (ZAR1) Validated Antibodies • Rabbit polyclonal anti-ZAR1 antibody Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • ZAR1 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • ZAR1 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Zar1 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • ZAR1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Zar1 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene ZAR1

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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