| Species: | Human |
Expression Host: | Human 293HEK cells |
| Expression cDNA Clone and Sequence |
Recombinant protein was produced with TrueORF clone, RC220457. Click on the TrueORF clone link to view cDNA and protein sequences. |
| Tag: | C-terminal MYC/DDK |
Predicted MW: | 288.7 kDa |
| Purity: | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Mass Spec Validation: |
This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. |
| Concentration: | >50 ug/mL as determined by microplate BCA method |
| Buffer and Storage: | 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. |
| Preparation: |
Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| BioActivity: |
MTOR activity verified in a biochemical assay: MTOR (mechanistic target of rapamycin) (TP320457) activity was measured in a homogeneous time-resolved fluorescent (HTRF®) assay. MTOR is a serine/threonine protein kinase that regulates cell growth, cell proliferation, cell motility, cell survival, protein synthesis, and transcription. MTOR belongs to the phosphatidylinositol 3-kinase-related kinase protein family. Varying concentrations of MTOR were added to a reaction mix containing ATP and a biotinylated kinase substrate and the reaction mixture was incubated to allow the protein to phosphorylate the substrate. HTRF detection reagents were then added, and the time-resolved fluorescent signal was measured on a Flexstation 3 microplate reader. The time resolved fluorescent signal is expressed as “delta R” or “ΔR” and is a ratio calculated from the fluorescent emission intensities of the donor and acceptor fluors.
MTOR activity was measured using the HTRF KinEASE® assay from Cis-Bio International. |
| Protein Families: |
Protein KinaseDruggable Genome |
| Protein Pathways: |
ErbB signaling pathwaymTOR signaling pathwayInsulin signaling pathwayAdipocytokine signaling pathwayType II diabetes mellitusPathways in cancerMore Pathways >> |
