|Expression cDNA Clone and Sequence
Recombinant protein was produced with TrueORF clone, RC220457. Click on the TrueORF clone link to view cDNA and protein sequences.|
||Predicted MW:||288.7 kDa|
|Purity:||> 80% as determined by SDS-PAGE and Coomassie blue staining|
|Concentration:||>50 ug/mL as determined by microplate BCA method|
|Buffer and Storage:||25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.|
||Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
||MTOR activity verified in a biochemical assay: MTOR (mechanistic target of rapamycin) (TP320457) activity was measured in a homogeneous time-resolved fluorescent (HTRF®) assay. MTOR is a serine/threonine protein kinase that regulates cell growth, cell proliferation, cell motility, cell survival, protein synthesis, and transcription. MTOR belongs to the phosphatidylinositol 3-kinase-related kinase protein family. Varying concentrations of MTOR were added to a reaction mix containing ATP and a biotinylated kinase substrate and the reaction mixture was incubated to allow the protein to phosphorylate the substrate. HTRF detection reagents were then added, and the time-resolved fluorescent signal was measured on a Flexstation 3 microplate reader. The time resolved fluorescent signal is expressed as “delta R” or “ΔR” and is a ratio calculated from the fluorescent emission intensities of the donor and acceptor fluors.
MTOR activity was measured using the HTRF KinEASE® assay from Cis-Bio International.
||Protein KinaseDruggable Genome
||ErbB signaling pathwaymTOR signaling pathwayInsulin signaling pathwayAdipocytokine signaling pathwayType II diabetes mellitusPathways in cancerMore Pathways >>