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Home Recombinant Human Proteins All MTOR Proteins

MTOR (NM_004958) Purified Human Protein

Specifications Citations Customer Service Product Documents
Cat. No. Description Datasheet Price Availability*  
TP320457 Recombinant protein of human FK506 binding protein 12-rapamycin associated protein 1 (FRAP1/MTOR), 20 ug (Larger pack size?) $680 In Stock
TA310067 Rabbit anti-MTOR polyclonal antibody $325 3 Weeks
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OriGene Data
Species:Human Expression Host:HEK293
Expression cDNA Clone or AA Sequence
Recombinant protein was produced with TrueORF clone, RC220457. Click on the TrueORF clone link to view cDNA and protein sequences.
Tag:C-terminal MYC/DDK Predicted MW:288.7 kDa
Purity:> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration:>50 ug/mL as determined by microplate BCA method
Buffer:25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
BioActivity: MTOR activity verified in a biochemical assay: MTOR (mechanistic target of rapamycin) (TP320457) activity was measured in a homogeneous time-resolved fluorescent (HTRF®) assay. MTOR is a serine/threonine protein kinase that regulates cell growth, cell proliferation, cell motility, cell survival, protein synthesis, and transcription. MTOR belongs to the phosphatidylinositol 3-kinase-related kinase protein family. Varying concentrations of MTOR were added to a reaction mix containing ATP and a biotinylated kinase substrate and the reaction mixture was incubated to allow the protein to phosphorylate the substrate. HTRF detection reagents were then added, and the time-resolved fluorescent signal was measured on a Flexstation 3 microplate reader. The time resolved fluorescent signal is expressed as “delta R” or “ΔR” and is a ratio calculated from the fluorescent emission intensities of the donor and acceptor fluors.

MTOR activity was measured using the HTRF KinEASE® assay from Cis-Bio International.
Protein Families: Protein KinaseDruggable Genome
Protein Pathways: ErbB signaling pathwaymTOR signaling pathwayInsulin signaling pathwayAdipocytokine signaling pathwayType II diabetes mellitusPathways in cancerMore Pathways >>
Protein Data
Protein Image
BioActivity Data
BioActivity Image
 

Reference Data
RefSeq: NP_004949 RefSeq Size: 8680 RefSeq ORF: 7650
LocusID: 2475 Cytogenetic: 1p36.2  
Synonyms : FRAP; FRAP1; FRAP2; RAFT1; RAPT1
Summary: The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq, Sep 2008].
*: Inventory for certain proteins may be limited due to low expression level. Delivery time may vary from web posted schedule. Contact techsupport@origene.com for specific inventory information
**: DDK-tag is the same as FLAG tag. Flag® is a registered trademark of Sigma-Aldrich

 

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