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OriGene protein in recent publications
Anti-TIF1 antibodies (anti-p155) in adult patients with dermatomyositis: comparison of different diagnostic assays Ann Rheum Dis, Jan 2012; 10.1136/annrheumdis-2011-200871. [TRIM33 ]
Novel p65 Binding Glucocorticoid-induced Leucine Zipper Peptide Suppresses Experimental Autoimmune Encephalomyelitis J. Biol. Chem., Dec 2011; 286: 44799 - 44810. [RELA]
Inhibitory Member of the Apoptosis-stimulating Proteins of the p53 Family (iASPP) Interacts with Protein Phosphatase 1 via a Noncanonical Binding Motif J. Biol. Chem., Dec 2011; 286: 43039 - 43044. [PPP1CB]
Phospholipase D2 (PLD2) is a guanine nucleotide exchange factor (GEF) for the GTPase Rac2 PNAS, Dec 2011; 108: 19617 - 19622. [VAV1]
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JAK3 (NM_000215) Purified Human Protein

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Cat. No. Ref. ID Description Price Availability*
TP317928 NM_000215 Purified recombinant protein of Homo sapiens Janus kinase 3 (a protein tyrosine kinase, leukocyte) (JAK3), expressed in human cells, 20 µg $680 Next Day

Also for JAK3 (NM_000215)
cDNA Clone
shRNA/siRNA
Mass Spec Std
Lysate
Antibody

OriGene Data
Expression Host:Human HEK293 cells Predicted MW:124.9 kDa
Protein Image Not Available
Expression cDNA Clone and Sequence HEK293 cells transient transfection with TrueORF clone, RC217928. Click on the TrueORF clone link to view cDNA clone sequence and protein sequence
Tag:C-terminal MYC/DDK** Anti-Myc/DDK antibody
Purity:> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration:> 50 ug/ml as determined by BCA
Preparation:HEK293T cells were transiently transfected with MegaTran Transfection Reagent (TT200002) and TrueORF cDNA plasmid. Transfected cells were cultured for 48-72 hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Recombinant proteins were purified through anti-DDK affinity columns. Protein concentration was measured by BCA kit.
Buffer and Storage:10% glycerol, 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.
Protein Families: Protein KinaseDruggable Genome
Protein Pathways: Chemokine signaling pathwayJak-STAT signaling pathwayPrimary immunodeficiency

Reference Data
RefSeq: NP_000206
 RefSeq Size: 4025 RefSeq ORF: 3375
Synonyms : JAK-3; JAK3_HUMAN; JAKL; L-JAK; LJAK
LocusID: 3718 Cytogenetic: 19p13.1  
Summary: The protein encoded by this gene is a member of the Janus kinase (JAK) family of tyrosine kinases involved in cytokine receptor-mediated intracellular signal transduction. It is predominantly expressed in immune cells and transduces a signal in response to its activation via tyrosine phosphorylation by interleukin receptors. Mutations in this gene are associated with autosomal SCID (severe combined immunodeficiency disease). [provided by RefSeq].
*: Inventory for certain proteins may be limited due to low expression level. Delivery time may vary from web posted schedule. Contact techsupport@origene.com for specific inventory information
**: DDK-tag is the same as FLAG tag. Flag® is a registered trademark of Sigma-Aldrich