AKT2 (NM_001626) Purified Human Protein - Active
AKT2 activity verified in a biochemical assay
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Also for AKT2 (NM_001626)
OriGene Data
| Expression Host: | Human HEK293 cells |
Predicted MW: | 55.6 kDa |
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| Expression cDNA Clone and Sequence |
HEK293 cells transient transfection with TrueORF clone, RC217733. Click on the TrueORF clone link to view cDNA clone sequence and protein sequence |
| Tag: | C-terminal MYC/DDK** Anti-Myc/DDK antibody |
| Purity: | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Mass Spec Validation: |
This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. |
| Bioactivity: |
AKT2 activity verified in a biochemical assay, view data |
| Concentration: | > 50 ug/ml as determined by BCA |
| Preparation: | HEK293T cells were transiently transfected with MegaTran Transfection Reagent (TT200002) and TrueORF cDNA plasmid. Transfected cells were cultured for 48-72 hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Recombinant proteins were purified through anti-DDK affinity columns. Protein concentration was measured by BCA kit. |
| Buffer and Storage: | 10% glycerol, 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Protein Families: |
Protein KinaseES Cell Differentiation/IPSDruggable Genome |
| Protein Pathways: |
MAPK signaling pathwayErbB signaling pathwayChemokine signaling pathwaymTOR signaling pathwayApoptosisVEGF signaling pathwayMore Pathways >> |
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Reference Data
RefSeq: NP_001617
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RefSeq Size: 1715 |
RefSeq ORF: 1446 |
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Synonyms : PKBB; PKBBETA; PRKBB; RAC-BETA
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| LocusID: 208 |
Cytogenetic: 19q13.1 |
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Summary: This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins. [provided by RefSeq].
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*: Inventory for certain proteins may be limited due to low expression level. Delivery time may vary from web posted schedule. Contact
techsupport@origene.com for specific inventory information
**: DDK-tag is the same as FLAG tag. Flag® is a registered trademark of Sigma-Aldrich
AKT2 (v-akt murine thymoma viral oncogene homolog 2) (TP317733) activity was measured in a homogeneous time-resolved fluorescent (HTRF®) assay. AKT2 is a serine/threonine kinase that plays a key in regulating cell survival, insulin signaling, angiogenesis and tumor formation. Varying concentrations of AKT2 were added to a reaction mix containing ATP and a biotinylated kinase substrate and the reaction mixture was incubated to allow the protein to phosphorylate the substrate. HTRF detection reagents were then added, and the time-resolved fluorescent signal was measured on a Flexstation 3 microplate reader. The time resolved fluorescent signal is expressed as “delta R” or “ΔR” and is a ratio calculated from the fluorescent emission intensities of the donor and acceptor fluors.
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