EGLN1 (NM_022051) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC215158. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 45.8 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Protein Pathways: Pathways in cancerRenal cell carcinoma

Protein Data

RefSeq: NP_071334	RefSeq Size: 5163	RefSeq ORF: 1281
LocusID: 54583	Cytogenetic: 1q42.1
Synonyms : C1orf12; ECYT3; HIF-PH2; HIFPH2; HPH-2; HPH2; PHD2; SM20; ZMYND6
Summary: The protein encoded by this gene catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. HIF is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. This protein functions as a cellular oxygen sensor, and under normal oxygen concentration, modification by prolyl hydroxylation is a key regulatory event that targets HIF subunits for proteasomal destruction via the von Hippel-Lindau ubiquitylation complex. Mutations in this gene are associated with erythrocytosis familial type 3 (ECYT3). [provided by RefSeq, Nov

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Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens egl nine homolog 1 (C. elegans) (EGLN1) as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens egl nine homolog 1 (C. elegans) (EGLN1) as transfection-ready DNA • Myc-DDK-tagged ORF clone of Homo sapiens egl nine homolog 1 (C. elegans) (EGLN1) as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of egl nine homolog 1 (C. elegans) (EGLN1) Validated Antibodies • Rabbit Polyclonal Antibody against HIF Prolyl Hydroxylase 2 • Rabbit monoclonal antibody against PHD2(clone EPR3661) • Mouse Monoclonal HIF Prolyl Hydroxylase 2 Antibody (366G/76/3) Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • EGLN1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • EGLN1 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • EGLN1 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene EGLN1

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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