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OriGene protein in recent publications
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Novel p65 Binding Glucocorticoid-induced Leucine Zipper Peptide Suppresses Experimental Autoimmune Encephalomyelitis J. Biol. Chem., Dec 2011; 286: 44799 - 44810. [RELA]
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AK1 (NM_000476) Purified Human Protein

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Cat. No. Ref. ID Description Price Availability*
TP315130 NM_000476 Purified recombinant protein of Homo sapiens adenylate kinase 1 (AK1), expressed in human cells, 20 µg $680 Next day

Also for AK1 (NM_000476)
cDNA Clone
shRNA/siRNA
Mass Spec Std
Lysate
Antibody

OriGene Data
Expression Host:Human HEK293 cells Predicted MW:21.5 kDa
Protein Image Not Available
Expression cDNA Clone and Sequence HEK293 cells transient transfection with TrueORF clone, RC215130. Click on the TrueORF clone link to view cDNA clone sequence and protein sequence
Tag:C-terminal MYC/DDK** Anti-Myc/DDK antibody
Purity:> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration:> 50 ug/ml as determined by BCA
Preparation:HEK293T cells were transiently transfected with MegaTran Transfection Reagent (TT200002) and TrueORF cDNA plasmid. Transfected cells were cultured for 48-72 hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Recombinant proteins were purified through anti-DDK affinity columns. Protein concentration was measured by BCA kit.
Buffer and Storage:10% glycerol, 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.
Protein Families: Druggable Genome
Protein Pathways: Purine metabolismMetabolic pathways

Reference Data
RefSeq: NP_000467
 RefSeq Size: 2271 RefSeq ORF: 585
Synonyms : ATP-AMP transphosphorylase; OTTHUMP00000022217; OTTHUMP00000022218; myokinase; adenylate kinase 1
LocusID: 203 Cytogenetic: 9q34.1  
Summary: Adenylate kinase is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate group among adinine nucleotides. Three isozymes of adenylate kinase have been identified in vertebrates, adenylate isozyme 1 (AK1), 2 (AK2) and 3 (AK3). AK1 is found in the cytosol of skeletal muscle, brain and erythrocytes, whereas AK2 and AK3 are found in the mitochondria of other tissues including liver and heart. AK1 was identified because of its association with a rare genetic disorder causing nonspherocytic hemolytic anemia where a mutation in the AK1 gene was found to reduce the catalytic activity of the enzyme. [provided by RefSeq].
*: Inventory for certain proteins may be limited due to low expression level. Delivery time may vary from web posted schedule. Contact techsupport@origene.com for specific inventory information
**: DDK-tag is the same as FLAG tag. Flag® is a registered trademark of Sigma-Aldrich