EDDM3B (NM_022360) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC213305. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 17.4 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Secreted ProteinTransmembrane Protein Pathways:

Protein Data

RefSeq: NP_071755	RefSeq Size: 909	RefSeq ORF: 444
LocusID: 64184	Cytogenetic: 14q11.2
Synonyms : EP3B; FAM12B; HE3-BETA; HE3B
Summary: Testicular sperm are morphologically differentiated but are not progressively motile nor able to fertilize an egg. Post-testicular maturation requires exposure of spermatozoa to the microenvironment of the epididymal lumen. Spermatozoa undergo extensive changes in the epididymis, including enzymatic modifications, loss of pre-existing components and addition of new glycoproteins from epididymal secretions. These modifying proteins and enzymes are synthesized by epithelial cells lining the epididymal duct and secreted apically into the lumen, where they come into contact with, and may be absorbed onto, the sperm membranes. The proteins encoded by the genes in this cluster are synthesized and secreted by epididymal epithelial cells. [provided by RefSeq, Jul 2008].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens epididymal protein 3B (EDDM3B) as transfection-ready DNA • Lenti ORF clone of Human epididymal protein 3B (EDDM3B) , Myc-DDK-tagged • Lenti ORF clone of Human epididymal protein 3B (EDDM3B), mGFP tagged • GFP-tagged ORF clone of Homo sapiens epididymal protein 3B (EDDM3B) as transfection-ready DNA • Myc-DDK-tagged ORF clone of Homo sapiens epididymal protein 3B (EDDM3B) as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of epididymal protein 3B (EDDM3B) Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • EDDM3B - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • EDDM3B - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Eddm3b - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • EDDM3B - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Gm75 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene EDDM3B

If you would like OriGene Technologies to prepare a purified protein in the quantity of your own interest, simply fill out the form and click Submit. OriGene will contact you.

Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
* Quantity (µg)
Comments
* First Name
* Last Name
* Email Address
* Institution Name
* Country
* Phone