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OriGene protein in recent publications
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Novel p65 Binding Glucocorticoid-induced Leucine Zipper Peptide Suppresses Experimental Autoimmune Encephalomyelitis J. Biol. Chem., Dec 2011; 286: 44799 - 44810. [RELA]
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AGXT (NM_000030) Purified Human Protein

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Cat. No. Ref. ID Description Price Availability*
TP312899 NM_000030 Purified recombinant protein of Homo sapiens alanine-glyoxylate aminotransferase (AGXT), expressed in human cells, 20 µg $680 Next day

Also for AGXT (NM_000030)
cDNA Clone
shRNA/siRNA
Mass Spec Std
Lysate
Antibody Service

OriGene Data
Expression Host:Human HEK293 cells Predicted MW:42.8 kDa
Protein Image Not Available
Expression cDNA Clone and Sequence HEK293 cells transient transfection with TrueORF clone, RC212899. Click on the TrueORF clone link to view cDNA clone sequence and protein sequence
Tag:C-terminal MYC/DDK** Anti-Myc/DDK antibody
Purity:> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration:> 50 ug/ml as determined by BCA
Preparation:HEK293T cells were transiently transfected with MegaTran Transfection Reagent (TT200002) and TrueORF cDNA plasmid. Transfected cells were cultured for 48-72 hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Recombinant proteins were purified through anti-DDK affinity columns. Protein concentration was measured by BCA kit.
Buffer and Storage:10% glycerol, 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.
Protein Families: Druggable Genome
Protein Pathways: Alanine, aspartate and glutamate metabolismGlycine, serine and threonine metabolismMetabolic pathways

Reference Data
RefSeq: NP_000021
 RefSeq Size: 1600 RefSeq ORF: 1179
Synonyms : AGT; AGT1; AGXT1; PH1; SPAT; SPT; TLH6
LocusID: 189 Cytogenetic: 2q37.3  
Summary: This gene is expressed only in the liver and the encoded protein is localized mostly in the peroxisomes, where it is involved in glyoxylate detoxification. Mutations in this gene, some of which alter subcellular targetting, have been associated with type I primary hyperoxaluria. [provided by RefSeq].
*: Inventory for certain proteins may be limited due to low expression level. Delivery time may vary from web posted schedule. Contact techsupport@origene.com for specific inventory information
**: DDK-tag is the same as FLAG tag. Flag® is a registered trademark of Sigma-Aldrich