L1CAM (NM_000425) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC211601. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 137.8 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: ES Cell Differentiation/IPSTransmembraneDruggable Genome Protein Pathways: Axon guidanceCell adhesion molecules (CAMs)

Protein Data

RefSeq: NP_000416	RefSeq Size: 4525	RefSeq ORF: 3774
LocusID: 3897	Cytogenetic: Xq28
Synonyms : CAML1; CD171; HSAS; HSAS1; MASA; MIC5; N-CAM-L1; N-CAML1; NCAM-L1; S10; SPG1
Summary: The protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause three X-linked neurological syndromes known by the acronym CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of a neuron-specific exon is thought to be functionally relevant. [provided by RefSeq, Jul 2008].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens L1 cell adhesion molecule (L1CAM), transcript variant 1 as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens L1 cell adhesion molecule (L1CAM), transcript variant 1 as transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of L1 cell adhesion molecule (L1CAM), transcript variant 1 Validated Antibodies • Rabbit polyclonal CD171/N-CAML1 (Ab-1181) antibody • Purified anti-L1CAM mouse monoclonal antibody, clone 2A6 • Purified L1CAM mouse monoclonal antibody, clone 2C7 • Purified L1CAM mouse monoclonal antibody, clone 2H5 • Purified L1CAM mouse monoclonal antibody, clone 3G1 • Purified L1CAM mouse monoclonal antibody, clone 4F4 • Purified L1CAM mouse monoclonal antibody, clone 10C12 • Purified L1CAM mouse monoclonal antibody, clone 1H3 • Purified L1CAM mouse monoclonal antibody, clone 2A11 • Purified L1CAM mouse monoclonal antibody, clone 1A8 • Purified L1CAM mouse monoclonal antibody, clone 2A5 • Purified L1CAM mouse monoclonal antibody, clone 1G4 • Purified L1CAM mouse monoclonal antibody, clone 1D12 • Purified anti-L1CAM mouse monoclonal antibody, clone 2G9 • Purified L1CAM mouse monoclonal antibody, clone 2G5 • Purified L1CAM mouse monoclonal antibody, clone 2H4 • Purified L1CAM mouse monoclonal antibody, clone 2B11 • Purified L1CAM mouse monoclonal antibody, clone 2H6 • Carrier-free (BSA/glycerol-free) L1CAM mouse monoclonal antibody, clone 2H6 • Carrier-free (BSA/glycerol-free) L1CAM mouse monoclonal antibody, clone 1H3 • Carrier-free (BSA/glycerol-free) L1CAM mouse monoclonal antibody, clone 2A6 • Carrier-free (BSA/glycerol-free) L1CAM mouse monoclonal antibody, clone 2C7 • Carrier-free (BSA/glycerol-free) L1CAM mouse monoclonal antibody, clone 2H5 • Carrier-free (BSA/glycerol-free) L1CAM mouse monoclonal antibody, clone 2A11 • Carrier-free (BSA/glycerol-free) L1CAM mouse monoclonal antibody, clone 1A8 • Carrier-free (BSA/glycerol-free) L1CAM mouse monoclonal antibody, clone 2A5 • Carrier-free (BSA/glycerol-free) L1CAM mouse monoclonal antibody, clone 1G4 • Carrier-free (BSA/glycerol-free) L1CAM mouse monoclonal antibody, clone 1D12 • Carrier-free (BSA/glycerol-free) L1CAM mouse monoclonal antibody, clone 2G9 • Carrier-free (BSA/glycerol-free) L1CAM mouse monoclonal antibody, clone 2G5 • Carrier-free (BSA/glycerol-free) L1CAM mouse monoclonal antibody, clone 2H4 • Carrier-free (BSA/glycerol-free) L1CAM mouse monoclonal antibody, clone 2B11 • Carrier-free (BSA/glycerol-free) L1CAM mouse monoclonal antibody, clone 3G1 • Carrier-free (BSA/glycerol-free) L1CAM mouse monoclonal antibody, clone 4F4 • Carrier-free (BSA/glycerol-free) L1CAM mouse monoclonal antibody, clone 10C12 • Purified L1CAM mouse monoclonal antibody, clone UMAB47 • Purified L1CAM mouse monoclonal antibody, clone UMAB48 • Purified L1CAM mouse monoclonal antibody, clone UMAB47 • Purified L1CAM mouse monoclonal antibody, clone UMAB48 Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • L1CAM - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • L1CAM - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • L1cam - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • L1CAM - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • L1cam - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene L1CAM

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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