CYP2C19 (NM_000769) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC211377. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 55.8 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Druggable Genome Protein Pathways: Arachidonic acid metabolismLinoleic acid metabolismRetinol metabolismMetabolism of xenobiotics by cytochrome P450Drug metabolism - cytochrome P450Metabolic pathways

Protein Data

RefSeq: NP_000760	RefSeq Size: 1473	RefSeq ORF: 1473
LocusID: 1557	Cytogenetic: 10q24
Synonyms : CPCJ; CYP2C; P450C2C; P450IIC19
Summary: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is known to metabolize many xenobiotics, including the anticonvulsive drug mephenytoin, omeprazole, diazepam and some barbiturates. Polymorphism within this gene is associated with variable ability to metabolize mephenytoin, known as the poor metabolizer and extensive metabolizer phenotypes. The gene is located within a cluster of cytochrome P450 genes on chromosome 10q24. [provided by RefSeq, Jul 2008].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens cytochrome P450, family 2, subfamily C, polypeptide 19 (CYP2C19) as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens cytochrome P450, family 2, subfamily C, polypeptide 19 (CYP2C19) as transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of cytochrome P450, family 2, subfamily C, polypeptide 19 (CYP2C19) Validated Antibodies • Rabbit polyclonal Cytochrome P450 2C19 antibody Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • CYP2C19 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • CYP2C19 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Cyp2c29 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • CYP2C19 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Cyp2c29 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene CYP2C19

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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