PF4 (NM_002619) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC211322. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 10.7 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Secreted ProteinTransmembraneDruggable Genome Protein Pathways: Cytokine-cytokine receptor interactionChemokine signaling pathway

Protein Data

RefSeq: NP_002610	RefSeq Size: 439	RefSeq ORF: 306
LocusID: 5196	Cytogenetic: 4q12
Synonyms : CXCL4; PF-4; SCYB4
Summary: This gene encodes a member of the CXC chemokine family. This chemokine is released from the alpha granules of activated platelets in the form of a homotetramer which has high affinity for heparin and is involved in platelet aggregation. This protein is chemotactic for numerous other cell type and also functions as an inhibitor of hematopoiesis, angiogenesis and T-cell function. [provided by RefSeq, Mar 2012].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens platelet factor 4 (PF4) as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens platelet factor 4 (PF4) as transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of platelet factor 4 (PF4) Validated Antibodies • Rabbit Monoclonal antibody against PF4 • Rabbit Monoclonal antibody against PF4 Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • PF4 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • PF4 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Pf4 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • PF4 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Cxcl4 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene PF4

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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