CST3 (NM_000099) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC210730. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 13.3 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: ES Cell Differentiation/IPSTransmembraneDruggable Genome Protein Pathways:

Protein Data

RefSeq: NP_000090	RefSeq Size: 818	RefSeq ORF: 441
LocusID: 1471	Cytogenetic: 20p11.21
Synonyms : ARMD11
Summary: The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes the most abundant extracellular inhibitor of cysteine proteases, which is found in high concentrations in biological fluids and is expressed in virtually all organs of the body. A mutation in this gene has been associated with amyloid angiopathy. Expression of this protein in vascular wall smooth muscle cells is severely reduced in both atherosclerotic and aneurysmal aortic lesions, establishing its role in vascular disease. [provided by RefSeq, Jul 2008].

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Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens cystatin C (CST3) as transfection-ready DNA • Lenti ORF clone of Human cystatin C (CST3), mGFP tagged • Lenti ORF clone of Human cystatin C (CST3) , Myc-DDK-tagged • GFP-tagged ORF clone of Homo sapiens cystatin C (CST3) as transfection-ready DNA • Myc-DDK-tagged ORF clone of Homo sapiens cystatin C (CST3) as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of cystatin C (CST3) Validated Antibodies • Rabbit Monoclonal antibody against Cystatin C (CST3) • Rabbit Monoclonal antibody against Cystatin C (CST3) • Goat Anti-CST3 / cystatin C Antibody • Purified CST3 (Cystatin C) mouse monoclonal antibody, Clone 2C8 • Purified CST3 (Cystatin C) mouse monoclonal antibody, Clone 2C11 • Purified CST3 (Cystatin C) mouse monoclonal antibody, Clone 1A6 • Purified CST3 (Cystatin C) mouse monoclonal antibody, Clone 7G8 • Purified CST3 (Cystatin C) mouse monoclonal antibody, Clone 7B8 • Rabbit polyclonal anti-CST3 antibody • Luminex validated purified CST3 mouse monoclonal antibody (clone 2C8), capture • Luminex validated purified CST3 mouse monoclonal antibody (clone 2C11), capture • Luminex validated purified CST3 mouse monoclonal antibody (clone 1A6), capture • Luminex validated purified CST3 mouse monoclonal antibody (clone 7G8), capture • Luminex validated purified CST3 mouse monoclonal antibody (clone 7B8), capture • Luminex validated purified biotinylated CST3 mouse monoclonal antibody (clone 2C8), detection • Luminex validated purified biotinylated CST3 mouse monoclonal antibody (clone 2C11), detection • Luminex validated purified biotinylated CST3 mouse monoclonal antibody (clone 1A6), detection • Luminex validated purified biotinylated CST3 mouse monoclonal antibody (clone 7G8), detection • Luminex validated purified biotinylated CST3 mouse monoclonal antibody (clone 7B8), detection • Carrier-free (BSA/glycerol-free) CST3 mouse monoclonal antibody,clone 2C8 • Carrier-free (BSA/glycerol-free) CST3 mouse monoclonal antibody,clone 2C11 • Carrier-free (BSA/glycerol-free) CST3 mouse monoclonal antibody,clone 1A6 • Carrier-free (BSA/glycerol-free) CST3 mouse monoclonal antibody,clone 7G8 • Carrier-free (BSA/glycerol-free) CST3 mouse monoclonal antibody,clone 7B8 Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • CST3 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • CST3 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Cst3 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • CST3 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Cst3 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene CST3

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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