H2AFZ (NM_002106) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC210691. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 13.4 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Druggable Genome Protein Pathways: Systemic lupus erythematosus

Protein Data

RefSeq: NP_002097	RefSeq Size: 951	RefSeq ORF: 387
LocusID: 3015	Cytogenetic: 4q24
Synonyms : H2A.z; H2A/z; H2AZ
Summary: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent member of the histone H2A family that is distinct from other members of the family. Studies in mice have shown that this particular histone is required for embryonic development and indicate that lack of functional histone H2A leads to embryonic lethality. [provided by RefSeq, Jul 2008].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens H2A histone family, member Z (H2AFZ) as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens H2A histone family, member Z (H2AFZ) as transfection-ready DNA • Myc-DDK-tagged ORF clone of Homo sapiens H2A histone family, member Z (H2AFZ) as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of H2A histone family, member Z (H2AFZ) Validated Antibodies • Rabbit Polyclonal antibody to Histone H2A.Z (H2A histone family, member Z) • Rabbit Polyclonal antibody to Histone H2A.Z (H2A histone family, member Z) Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • H2AFZ - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • H2AFZ - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • H2afz - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • H2AFZ - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • H2afz - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene H2AFZ

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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