WEE1 (NM_003390) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC209760. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 71.4 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Protein KinaseStem cell - PluripotencyDruggable Genome Protein Pathways: Cell cycle

Protein Data

RefSeq: NP_003381	RefSeq Size: 4232	RefSeq ORF: 1941
LocusID: 7465	Cytogenetic: 11p15.3
Synonyms : WEE1A; WEE1hu
Summary: This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase. [provided by RefSeq, Jul

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Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens WEE1 homolog (S. pombe) (WEE1), transcript variant 1 as transfection-ready DNA • Lenti ORF clone of Human WEE1 homolog (S. pombe) (WEE1), transcript variant 1 , Myc-DDK-tagged • Lenti ORF clone of Human WEE1 homolog (S. pombe) (WEE1), transcript variant 1, mGFP tagged • GFP-tagged ORF clone of Homo sapiens WEE1 homolog (S. pombe) (WEE1), transcript variant 1 as transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of WEE1 homolog (S. pombe) (WEE1), transcript variant 1 Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • WEE1 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • WEE1 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Wee1 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • WEE1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Wee1 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene WEE1

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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