VGF (NM_003378) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC209477. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 65 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Secreted Protein Protein Pathways:

Protein Data

RefSeq: NP_003369	RefSeq Size: 2545	RefSeq ORF: 1848
LocusID: 7425	Cytogenetic: 7q22.1
Synonyms : VGF nerve growth factor inducible; Gm1052
Summary: This gene is specifically expressed in a subpopulation of neuroendocrine cells, and is upregulated by nerve growth factor. The structural organization of this gene is similar to that of the rat gene, and both the translated and the untranslated regions show a high degree of sequence similarity to the rat gene. The encoded secretory protein also shares similarities with the secretogranin/chromogranin family, however, its exact function is not known. [provided by RefSeq, Jul 2008].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens VGF nerve growth factor inducible (VGF) as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens VGF nerve growth factor inducible (VGF) as transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of VGF nerve growth factor inducible (VGF) Validated Antibodies • Rabbit Polyclonal VGF Antibody • Rabbit polyclonal anti-VGF antibody Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • VGF - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • VGF - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Vgf - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • VGF - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Vgf - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene VGF

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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