GALNS (NM_000512) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC208708. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 55.4 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Druggable Genome Protein Pathways: Glycosaminoglycan degradationMetabolic pathwaysLysosome

Protein Data

RefSeq: NP_000503	RefSeq Size: 2390	RefSeq ORF: 1569
LocusID: 2588	Cytogenetic: 16q24.3
Synonyms : GALNAC6S; GAS; MPS4A
Summary: This gene encodes N-acetylgalactosamine-6-sulfatase which is a lysosomal exohydrolase required for the degradation of the glycosaminoglycans, keratan sulfate, and chondroitin 6-sulfate. Sequence alterations including point, missense and nonsense mutations, as well as those that affect splicing, result in a deficiency of this enzyme. Deficiencies of this enzyme lead to Morquio A syndrome, a lysosomal storage disorder. [provided by RefSeq, Jul 2008].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens galactosamine (N-acetyl)-6-sulfate sulfatase (GALNS) as transfection-ready DNA • Lenti ORF clone of Human galactosamine (N-acetyl)-6-sulfate sulfatase (GALNS) , Myc-DDK-tagged • GFP-tagged ORF clone of Homo sapiens galactosamine (N-acetyl)-6-sulfate sulfatase (GALNS) as transfection-ready DNA • Lenti ORF clone of Human galactosamine (N-acetyl)-6-sulfate sulfatase (GALNS), mGFP tagged • Myc-DDK-tagged ORF clone of Homo sapiens galactosamine (N-acetyl)-6-sulfate sulfatase (GALNS) as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of galactosamine (N-acetyl)-6-sulfate sulfatase (GALNS) Validated Antibodies • Purified Goat Polyclonal Antibody against GALNS • Rabbit Polyclonal antibody to GALNS (galactosamine (N-acetyl)-6-sulfate sulfatase) Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • GALNS - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • GALNS - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Galns - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • GALNS - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Galns - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene GALNS

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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