PCBP1 (NM_006196) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC207878. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 37.3 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Protein Pathways: Spliceosome

Protein Data

RefSeq: NP_006187	RefSeq Size: 1634	RefSeq ORF: 1071
LocusID: 5093	Cytogenetic: 2p13
Synonyms : hnRNP-E1; hnRNP-X; HNRPE1; HNRPX
Summary: This intronless gene is thought to have been generated by retrotransposition of a fully processed PCBP-2 mRNA. This gene and PCBP-2 have paralogues (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. The protein encoded by this gene appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. [provided by RefSeq, Jul 2008].

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Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens poly(rC) binding protein 1 (PCBP1) as transfection-ready DNA • Lenti ORF clone of Human poly(rC) binding protein 1 (PCBP1) , Myc-DDK-tagged • GFP-tagged ORF clone of Homo sapiens poly(rC) binding protein 1 (PCBP1) as transfection-ready DNA • Lenti ORF clone of Human poly(rC) binding protein 1 (PCBP1), mGFP tagged • Myc-DDK-tagged ORF clone of Homo sapiens poly(rC) binding protein 1 (PCBP1) as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of poly(rC) binding protein 1 (PCBP1) Validated Antibodies • Goat Anti-PCBP1 (aa223-234) Antibody • Goat Anti-PCBP1 (aa234-247) Antibody Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • PCBP1 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • PCBP1 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Pcbp1 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • PCBP1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Pcbp1 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene PCBP1

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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