||Expression Host:||Human 293HEK cells
|Expression cDNA Clone and Sequence
Recombinant protein was produced with TrueORF clone, RC205821. Click on the TrueORF clone link to view cDNA and protein sequences.|
||Predicted MW:||85.5 kDa|
|Purity:||> 80% as determined by SDS-PAGE and Coomassie blue staining|
|Mass Spec Validation:
||This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology.
|Concentration:||>50 ug/mL as determined by microplate BCA method|
|Buffer and Storage:||25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.|
||Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
||TPX2 activity in a microtubule binding assay: SAF-A binds to MT in a TPX2-dependent manner. MT binding analysis of isolated SAF-A protein in the presence of different amounts of recombinant TPX2 protein. The lower panel shows western blotting with anti-SAF-A antibody. SAF-A co-pelleted with MT was increased, dependent on the amount of TPX2. S, supernatant; P,pellet.
Nan Ma, Sachihiro Matsunaga, Kiichi Fukui, et al. J. Cell Science. 124, 394-404. 2011.
||Stem cell - PluripotencyDruggable Genome