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Home Recombinant Human Proteins All ARG1 Proteins

ARG1 (NM_000045) Purified Human Protein

Specifications Citations Customer Service Product Documents
Cat. No. Description Datasheet Price Availability*  
TP304649 Recombinant protein of human arginase, liver (ARG1), 20 ug (Larger pack size?) $680 In Stock
TA502413 ARG1 (liver Arginase) mouse monoclonal antibody, clone 4E6 $379 Next Day
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OriGene Data
Species:Human Expression Host:HEK293
Expression cDNA Clone or AA Sequence
Recombinant protein was produced with TrueORF clone, RC204649. Click on the TrueORF clone link to view cDNA and protein sequences.
Tag:C-terminal MYC/DDK Predicted MW:34.6 kDa
Purity:> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration:>50 ug/mL as determined by microplate BCA method
Buffer:25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
BioActivity: Arginase activity verified in a biochemical assay: Arginase 1 (ARG1, TP304649) activity was measured in a colorimetric biochemical assay. Arginase 1 catalyzes the conversion of arginine to ornithine and urea. After incubation of the protein in a solution containing L-arginine, the reaction is stopped, and the urea concentration is measured by a chemical reaction that produces a colored product that absorbs at 430 nm. By measuring the absorbance at 430 nm and comparing to a standard, the specific activity of this preparation of ARG1 was calculated to be approximately 10U/mg.
Unit definition: 1 unit of ARG1 converts 1 µmole of L-arginine to ornithine and urea per minute at pH 9.5 and 37ºC.
Protein Families: Druggable Genome
Protein Pathways: Arginine and proline metabolismMetabolic pathways
Protein Data
Protein Image
BioActivity Data
BioActivity Image
 

Reference Data
RefSeq: NP_000036 RefSeq Size: 1447 RefSeq ORF: 969
LocusID: 383 Cytogenetic: 6q23  
Synonyms : OTTHUMP00000017209; arginase 1; liver-type arginase; type I arginase; arginase, liver
Summary: Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011].
*: Inventory for certain proteins may be limited due to low expression level. Delivery time may vary from web posted schedule. Contact techsupport@origene.com for specific inventory information
**: DDK-tag is the same as FLAG tag. Flag® is a registered trademark of Sigma-Aldrich

 

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