DAZAP1 (NM_018959) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC204091. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 43.2 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Stem cell - Pluripotency Protein Pathways:

Protein Data

RefSeq: NP_061832	RefSeq Size: 2185	RefSeq ORF: 1224
LocusID: 26528	Cytogenetic: 19p13.3
Synonyms : MGC19907
Summary: In mammals, the Y chromosome directs the development of the testes and plays an important role in spermatogenesis. A high percentage of infertile men have deletions that map to regions of the Y chromosome. The DAZ (deleted in azoospermia) gene cluster maps to the AZFc region of the Y chromosome and is deleted in many azoospermic and severely oligospermic men. It is thought that the DAZ gene cluster arose from the transposition, amplification, and pruning of the ancestral autosomal gene DAZL also involved in germ cell development and gametogenesis. This gene encodes a RNA-binding protein with two RNP motifs that was originally identified by its interaction with the infertility factors DAZ and DAZL. Two isoforms are encoded by transcript variants of this gene. [provided by RefSeq, Jul 2008].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens DAZ associated protein 1 (DAZAP1), transcript variant 2 as transfection-ready DNA • Lenti ORF clone of Human DAZ associated protein 1 (DAZAP1), transcript variant 2 , Myc-DDK-tagged • Lenti ORF clone of Human DAZ associated protein 1 (DAZAP1), transcript variant 2, mGFP tagged • GFP-tagged ORF clone of Homo sapiens DAZ associated protein 1 (DAZAP1), transcript variant 2 as transfection-ready DNA • Myc-DDK-tagged ORF clone of Homo sapiens DAZ associated protein 1 (DAZAP1), transcript variant 2 as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of DAZ associated protein 1 (DAZAP1), transcript variant 2 Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • DAZAP1 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • DAZAP1 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Dazap1 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • DAZAP1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Dazap1 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene DAZAP1

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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