C12orf5 (NM_020375) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC204087. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 29.9 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Protein Pathways:

Protein Data

RefSeq: NP_065108	RefSeq Size: 8237	RefSeq ORF: 813
LocusID: 57103	Cytogenetic: 12p13.3
Synonyms : FR2BP; TIGAR
Summary: This gene is regulated as part of the p53 tumor suppressor pathway and encodes a protein with sequence similarity to the bisphosphate domain of the glycolytic enzyme that degrades fructose-2,6-bisphosphate. The protein functions by blocking glycolysis and directing the pathway into the pentose phosphate shunt. Expression of this protein also protects cells from DNA damaging reactive oxygen species and provides some protection from DNA damage-induced apoptosis. The 12p13.32 region that includes this gene is paralogous to the 11q13.3 region. [provided by RefSeq, Jul 2008].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens chromosome 12 open reading frame 5 (C12orf5) as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens chromosome 12 open reading frame 5 (C12orf5) as transfection-ready DNA • Myc-DDK-tagged ORF clone of Homo sapiens chromosome 12 open reading frame 5 (C12orf5) as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of chromosome 12 open reading frame 5 (C12orf5) Validated Antibodies • Rabbit Polyclonal TIGAR Antibody • Rabbit Polyclonal TIGAR Antibody • TIGAR Rabbit Polyclonal (Internal) Antibody • TIGAR Rabbit Polyclonal (Internal) Antibody • Anti-CEA Rabbit Polyclonal Antibody • TIGAR Rabbit Polyclonal (aa140-190) Antibody Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • C12orf5 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • C12orf5 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • 9630033F20Rik - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • C12orf5 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • 9630033F20Rik - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene C12orf5

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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