RNH1 (NM_203388) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC203946. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 49.8 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Protein Pathways:

Protein Data

RefSeq: NP_976322	RefSeq Size: 1764	RefSeq ORF: 1386
LocusID: 6050	Cytogenetic: 11p15.5
Synonyms : RAI; RNH
Summary: Placental ribonuclease inhibitor (PRI) is a member of a family of proteinaceous cytoplasmic RNase inhibitors that occur in many tissues and bind to both intracellular and extracellular RNases (summarized by Lee et al., 1988 [PubMed 3219362]). In addition to control of intracellular RNases, the inhibitor may have a role in the regulation of angiogenin (MIM 105850). Ribonuclease inhibitor, of 50,000 Da, binds to ribonucleases and holds them in a latent form. Since neutral and alkaline ribonucleases probably play a critical role in the turnover of RNA in eukaryotic cells, RNH may be essential for control of mRNA turnover; the interaction of eukaryotic cells with ribonuclease may be reversible in vivo.[supplied by OMIM, Jul 2010].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens ribonuclease/angiogenin inhibitor 1 (RNH1), transcript variant 7 as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens ribonuclease/angiogenin inhibitor 1 (RNH1), transcript variant 7 as transfection-ready DNA • Myc-DDK-tagged ORF clone of Homo sapiens ribonuclease/angiogenin inhibitor 1 (RNH1), transcript variant 7 as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of ribonuclease/angiogenin inhibitor 1 (RNH1), transcript variant 7 Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • RNH1 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • RNH1 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Rnh1 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • RNH1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Rnh1 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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