TADA3 (NM_006354) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC203687. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 48.7 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Transcription Factors Protein Pathways:

Protein Data

RefSeq: NP_006345	RefSeq Size: 2245	RefSeq ORF: 1299
LocusID: 10474	Cytogenetic: 3p25.3
Synonyms : ADA3; hADA3; NGG1; TADA3L
Summary: Many DNA-binding transcriptional activator proteins enhance the initiation rate of RNA polymerase II-mediated gene transcription by interacting functionally with the general transcription machinery bound at the basal promoter. Adaptor proteins are usually required for this activation, possibly to acetylate and destabilize nucleosomes, thereby relieving chromatin constraints at the promoter. The protein encoded by this gene is a transcriptional activator adaptor and has been found to be part of the PCAF histone acetylase complex. In addition, it associates with the tumor suppressor protein p53 and is required for full activity of p53 and p53-mediated apoptosis. At least four alternatively spliced variants have been found for this gene, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens transcriptional adaptor 3 (TADA3), transcript variant 1 as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens transcriptional adaptor 3 (TADA3), transcript variant 1 as transfection-ready DNA • Lenti ORF clone of Human transcriptional adaptor 3 (TADA3), transcript variant 1 , Myc-DDK-tagged • Lenti ORF clone of Human transcriptional adaptor 3 (TADA3), transcript variant 1, mGFP tagged • Myc-DDK-tagged ORF clone of Homo sapiens transcriptional adaptor 3 (TADA3), transcript variant 1 as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of transcriptional adaptor 3 (TADA3), transcript variant 1 Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • TADA3 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • TADA3 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Tada3 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • TADA3L - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Tada3 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene TADA3

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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