BANF1 (NM_003860) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC203270. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 9.9 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Protein Pathways:

Protein Data

RefSeq: NP_003851	RefSeq Size: 1192	RefSeq ORF: 270
LocusID: 8815	Cytogenetic: 11q13.1
Synonyms : BAF; BCRP1; D14S1460; NGPS
Summary: The protein encoded by this gene was first identified by its ability to protect retroviruses from intramolecular integration and therefore promote intermolecular integration into the host cell genome. The protein forms a homodimer which localizes to both the nucleus and cytoplasm and is specifically associated with chromosomes during mitosis. This protein binds to double stranded DNA in a non-specific manner and also binds to LEM-domain containing proteins of the nuclear envelope. This protein is thought to facilitate nuclear reassembly by binding with both DNA and inner nuclear membrane proteins and thereby recruit chromatin to the nuclear periphery. Alternative splicing results in multiple transcript variants encoding the same protein.[provided by RefSeq, Jan 2009].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens barrier to autointegration factor 1 (BANF1), transcript variant 1 as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens barrier to autointegration factor 1 (BANF1), transcript variant 1 as transfection-ready DNA • Lenti ORF clone of Human barrier to autointegration factor 1 (BANF1), transcript variant 1 , Myc-DDK-tagged • Lenti ORF clone of Human barrier to autointegration factor 1 (BANF1), transcript variant 1, mGFP tagged • Myc-DDK-tagged ORF clone of Homo sapiens barrier to autointegration factor 1 (BANF1), transcript variant 1 as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of barrier to autointegration factor 1 (BANF1), transcript variant 1 Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • BANF1 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • BANF1 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Banf1 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • BANF1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Banf1 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene BANF1

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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