HBG1 (NM_000559) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC203084. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 15.9 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Protein Pathways:

Protein Data

RefSeq: NP_000550	RefSeq Size: 584	RefSeq ORF: 444
LocusID: 3047	Cytogenetic: 11p15.5
Synonyms : HBGA; HBGR; HSGGL1; PRO2979
Summary: The gamma globin genes (HBG1 and HBG2) are normally expressed in the fetal liver, spleen and bone marrow. Two gamma chains together with two alpha chains constitute fetal hemoglobin (HbF) which is normally replaced by adult hemoglobin (HbA) at birth. In some beta-thalassemias and related conditions, gamma chain production continues into adulthood. The two types of gamma chains differ at residue 136 where glycine is found in the G-gamma product (HBG2) and alanine is found in the A-gamma product (HBG1). The former is predominant at birth. The order of the genes in the beta-globin cluster is: 5'-epsilon -- gamma-G -- gamma-A -- delta -- beta--3'. [provided by RefSeq, Jul 2008].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens hemoglobin, gamma A (HBG1) as transfection-ready DNA • Lenti ORF clone of Human hemoglobin, gamma A (HBG1), mGFP tagged • Lenti ORF clone of Human hemoglobin, gamma A (HBG1) , Myc-DDK-tagged • GFP-tagged ORF clone of Homo sapiens hemoglobin, gamma A (HBG1) as transfection-ready DNA • Myc-DDK-tagged ORF clone of Homo sapiens hemoglobin, gamma A (HBG1) as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of hemoglobin, gamma A (HBG1) Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • HBG1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • HBG1 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • HBG1 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene HBG1

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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