GALT (NM_000155) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC202206. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 43.2 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Druggable Genome Protein Pathways: Galactose metabolismAmino sugar and nucleotide sugar metabolismMetabolic pathways

Protein Data

RefSeq: NP_000146	RefSeq Size: 1347	RefSeq ORF: 1140
LocusID: 2592	Cytogenetic: 9p13
Synonyms : OTTHUMP00000021298; galactose-1-phosphate uridyl transferase; galactose-1-phosphate uridylyltransferase
Summary: Galactose-1-phosphate uridyl transferase (GALT) catalyzes the second step of the Leloir pathway of galactose metabolism, namely the conversion of UDP-glucose + galactose-1-phosphate to glucose-1-phosphate + UDP-galactose. The absence of this enzyme results in classic galactosemia in humans and can be fatal in the newborn period if lactose is not removed from the diet. The pathophysiology of galactosemia has not been clearly defined. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012].

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Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens galactose-1-phosphate uridylyltransferase (GALT) as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens galactose-1-phosphate uridylyltransferase (GALT) as transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of galactose-1-phosphate uridylyltransferase (GALT) Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • GALT - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • GALT - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Galt - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • GALT - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Galt - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene GALT

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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