ODF2 (NM_002540) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC201985. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 93.1 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Protein Pathways:

Protein Data

RefSeq: NP_002531	RefSeq Size: 3772	RefSeq ORF: 2418
LocusID: 4957	Cytogenetic: 9q34.11
Synonyms : CT134; ODF2/1; ODF2/2; ODF84
Summary: The outer dense fibers are cytoskeletal structures that surround the axoneme in the middle piece and principal piece of the sperm tail. The fibers function in maintaining the elastic structure and recoil of the sperm tail as well as in protecting the tail from shear forces during epididymal transport and ejaculation. Defects in the outer dense fibers lead to abnormal sperm morphology and infertility. This gene encodes one of the major outer dense fiber proteins. Alternative splicing results in multiple transcript variants. The longer transcripts, also known as 'Cenexins', encode proteins with a C-terminal extension that are differentially targeted to somatic centrioles and thought to be crucial for the formation of microtubule organizing centers. [provided by RefSeq, Oct 2010].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens outer dense fiber of sperm tails 2 (ODF2), transcript variant 1 as transfection-ready DNA • Lenti ORF clone of Human outer dense fiber of sperm tails 2 (ODF2), transcript variant 1 , Myc-DDK-tagged • Lenti ORF clone of Human outer dense fiber of sperm tails 2 (ODF2), transcript variant 1, mGFP tagged • GFP-tagged ORF clone of Homo sapiens outer dense fiber of sperm tails 2 (ODF2), transcript variant 1 as transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of outer dense fiber of sperm tails 2 (ODF2), transcript variant 1 Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • ODF2 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • ODF2 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Odf2 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • ODF2 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Odf2 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene ODF2

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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