AKT1 (NM_001014431) Purified Human Protein
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OriGene Data
| Expression Host: | Human HEK293 cells |
Predicted MW: | 55.5 kDa |
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| Expression cDNA Clone and Sequence |
HEK293 cells transient transfection with TrueORF clone, RC201850. Click on the TrueORF clone link to view cDNA clone sequence and protein sequence |
| Tag: | C-terminal MYC/DDK** Anti-Myc/DDK antibody |
| Purity: | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Mass Spec Validation: |
This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. |
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| Concentration: | > 50 ug/ml as determined by BCA |
| Preparation: | HEK293T cells were transiently transfected with MegaTran Transfection Reagent (TT200002) and TrueORF cDNA plasmid. Transfected cells were cultured for 48-72 hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Recombinant proteins were purified through anti-DDK affinity columns. Protein concentration was measured by BCA kit. |
| Buffer and Storage: | 10% glycerol, 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Protein Families: |
Protein KinaseES Cell Differentiation/IPSDruggable Genome |
| Protein Pathways: |
MAPK signaling pathwayErbB signaling pathwayChemokine signaling pathwaymTOR signaling pathwayApoptosisVEGF signaling pathwayMore Pathways >> |
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Reference Data
RefSeq: NP_001014431
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RefSeq Size: 2794 |
RefSeq ORF: 1443 |
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Synonyms : AKT; MGC99656; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA
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| LocusID: 207 |
Cytogenetic: 14q32.32 |
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Summary: The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq].
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*: Inventory for certain proteins may be limited due to low expression level. Delivery time may vary from web posted schedule. Contact
techsupport@origene.com for specific inventory information
**: DDK-tag is the same as FLAG tag. Flag® is a registered trademark of Sigma-Aldrich
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