PPP1R15A (NM_014330) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC200581. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 73.3 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Druggable Genome Protein Pathways:

Protein Data

RefSeq: NP_055145	RefSeq Size: 2349	RefSeq ORF: 2025
LocusID: 23645	Cytogenetic: 19q13.2
Synonyms : GADD34
Summary: This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The induction of this gene by ionizing radiation occurs in certain cell lines regardless of p53 status, and its protein response is correlated with apoptosis following ionizing radiation. [provided by RefSeq, Jul 2008].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens protein phosphatase 1, regulatory (inhibitor) subunit 15A (PPP1R15A) as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens protein phosphatase 1, regulatory (inhibitor) subunit 15A (PPP1R15A) as transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of protein phosphatase 1, regulatory (inhibitor) subunit 15A (PPP1R15A) Validated Antibodies • Purified PPP1R15A (GADD34) mouse monoclonal antibody, clone 2B11 • Purified PPP1R15A (GADD34) mouse monoclonal antibody, clone 3D12 • Purified PPP1R15A (GADD34) mouse monoclonal antibody, clone 4F1 • Purified PPP1R15A (GADD34) mouse monoclonal antibody, clone 1E1 • Purified Goat Polyclonal Antibody against PPP1R15A • Carrier-free (BSA/glycerol-free) PPP1R15A mouse monoclonal antibody,clone 2B11 • Carrier-free (BSA/glycerol-free) PPP1R15A mouse monoclonal antibody,clone 3D12 • Carrier-free (BSA/glycerol-free) PPP1R15A mouse monoclonal antibody,clone 4F1 • Carrier-free (BSA/glycerol-free) PPP1R15A mouse monoclonal antibody,clone 1E1 Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • PPP1R15A - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • PPP1R15A - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Ppp1r15a - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • PPP1R15A - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Ppp1r15a - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene PPP1R15A

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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