ALDH2 (NM_000690) Purified Human Protein
|
|
OriGene Data
| Expression Host: | Human HEK293 cells |
Predicted MW: | 54.4 kDa |
|
| Expression cDNA Clone and Sequence |
HEK293 cells transient transfection with TrueORF clone, RC200505. Click on the TrueORF clone link to view cDNA clone sequence and protein sequence |
| Tag: | C-terminal MYC/DDK** Anti-Myc/DDK antibody |
| Purity: | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Mass Spec Validation: |
This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. |
|
|
| Concentration: | > 50 ug/ml as determined by BCA |
| Preparation: | HEK293T cells were transiently transfected with MegaTran Transfection Reagent (TT200002) and TrueORF cDNA plasmid. Transfected cells were cultured for 48-72 hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Recombinant proteins were purified through anti-DDK affinity columns. Protein concentration was measured by BCA kit. |
| Buffer and Storage: | 10% glycerol, 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Protein Families: |
Druggable Genome |
| Protein Pathways: |
Glycolysis / GluconeogenesisAscorbate and aldarate metabolismFatty acid metabolismValine, leucine and isoleucine degradationLysine degradationArginine and proline metabolismMore Pathways >> |
|
Reference Data
RefSeq: NP_000681
|
RefSeq Size: 2445 |
RefSeq ORF: 1554 |
|
Synonyms : ALDH-E2; ALDHI; ALDM; MGC1806
|
| LocusID: 217 |
Cytogenetic: 12q24.2 |
|
|
Summary: This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of aldehyde dehydrogenase, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have the cytosolic isozyme but not the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of a catalytically active form of the mitochondrial isozyme. The increased exposure to acetaldehyde in individuals with the catalytically inactive form may also confer greater susceptibility to many types of cancer. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
|
|
*: Inventory for certain proteins may be limited due to low expression level. Delivery time may vary from web posted schedule. Contact
techsupport@origene.com for specific inventory information
**: DDK-tag is the same as FLAG tag. Flag® is a registered trademark of Sigma-Aldrich
| Researchers who bought this clone have also purchased: |
|
Related Lysate from OriGene
Anti-Tag Antibodies & WB Reagent
Related Primer Pair
|
|