YWHAE (NM_006761) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC200245. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 29 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Druggable Genome Protein Pathways: Cell cycleOocyte meiosisNeurotrophin signaling pathway

Protein Data

RefSeq: NP_006752	RefSeq Size: 1810	RefSeq ORF: 768
LocusID: 7531	Cytogenetic: 17p13.3
Synonyms : 14-3-3E; KCIP-1; MDCR; MDS
Summary: This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene. [provided by RefSeq, Aug 2008].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide (YWHAE), transcript variant 1 as transfection-ready DNA • Lenti ORF clone of Human tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide (YWHAE), transcript variant 1 , Myc-DDK-tagged • Lenti ORF clone of Human tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide (YWHAE), transcript variant 1, mGFP tagged • GFP-tagged ORF clone of Homo sapiens tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide (YWHAE), transcript variant 1 as transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide (YWHAE), transcript variant 1 Validated Antibodies • Rabbit polyclonal anti-14-3-3 e antibody • Rabbit monoclonal antibody against 14-3-3E (clone EPR3918 ) • Rabbit monoclonal antibody against 14-3-3E(clone EPR3919) • 14-3-3 Epsilon Mouse Monoclonal (aa1-255) (5A5) Antibody • 14-3-3 Epsilon Rabbit anti-Sheep Polyclonal (N-Terminus) Antibody Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • YWHAE - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • YWHAE - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Ywhae - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • YWHAE - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Ywhae - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene YWHAE

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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