LMCD1 (NM_014583) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC200062. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 40.7 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Protein Pathways:

Protein Data

RefSeq: NP_055398	RefSeq Size: 1754	RefSeq ORF: 1098
LocusID: 29995	Cytogenetic: 3p26
Synonyms : dyxin; LIM and cysteine-rich domains 1
Summary: The protein encoded by this gene contains a cysteine-rich domain in the N-terminal region and 2 LIM domains in the C-terminal region. It also has several potential phosphorylation and N-myristoylation sites and a single potential N-glycosylation site. The presence of LIM domains implies involvement in protein-protein interactions. Expression of this gene has been detected in most tissues, with highest expression in skeletal muscle. Transcript variants utilizing alternative polyA signals have been observed. [provided by RefSeq, Jul 2008].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens LIM and cysteine-rich domains 1 (LMCD1) as transfection-ready DNA • Lenti ORF clone of Human LIM and cysteine-rich domains 1 (LMCD1), mGFP tagged • GFP-tagged ORF clone of Homo sapiens LIM and cysteine-rich domains 1 (LMCD1) as transfection-ready DNA • Lenti ORF clone of Human LIM and cysteine-rich domains 1 (LMCD1) , Myc-DDK-tagged • Myc-DDK-tagged ORF clone of Homo sapiens LIM and cysteine-rich domains 1 (LMCD1) as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of LIM and cysteine-rich domains 1 (LMCD1) Validated Antibodies • LMCD1 mouse monoclonal antibody, clone 2F12 • LMCD1 mouse monoclonal antibody, clone 2G5 • LMCD1 mouse monoclonal antibody,clone 1H5 • Carrier-free (BSA/glycerol-free) LMCD1 mouse monoclonal antibody, clone 2F12 • Carrier-free (BSA/glycerol-free) LMCD1 mouse monoclonal antibody, clone 2G5 • Carrier-free (BSA/glycerol-free) LMCD1 mouse monoclonal antibody,clone 1H5 Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • LMCD1 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • LMCD1 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Lmcd1 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • LMCD1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Lmcd1 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene LMCD1

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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