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OriGene cDNA clones in recent publications
Silencing hsp25/hsp27 Gene Expression Augments Proteasome Activity and Increases CD8+ T-Cell–Mediated Tumor Killing and Memory Responses Cancer Prevention Research, Jan 2012; 5: 122 - 137. [HSPB1 ]
Activation of Androgen Receptor, Lipogenesis, and Oxidative Stress Converged by SREBP-1 Is Responsible for Regulating Growth and Progression of Prostate Cancer Cells Mol. Cancer Res., Jan 2012; 10: 133 - 142. [SREBF1 ]
Androgen Deprivation Causes Epithelial–Mesenchymal Transition in the Prostate: Implications for Androgen-Deprivation Therapy Cancer Res., Jan 2012; 72: 527 - 536. [AR ]
CB2 Cannabinoid Receptors Promote Neural Progenitor Cell Proliferation via mTORC1 Signaling J. Biol. Chem., Jan 2012; 287: 1198 - 1209. [Cnr2 ]
View All Citations >>

Alpha-Gold Competent Cells

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OriGene’s choice for in-house transformation of DNA…now available to our customers

Alpha-Gold has been used for cloning & transformation of OriGene’s major plasmid products such as TrueORF Gold cDNA clones, HuSH-29 shRNA & MicroRNA expression plasmids.

Features:

  • Both Electroporation Grade and Chemically Competent
  • Comparable to DH5άTM in transformation efficiency
  • Accommodates small and larger plasmids
  • recA1 and endA1 markers for minimal recombination and enhanced quality of plasmid DNA

Genotype: F- deoR endA1 recA1 relA1 gyrA96 hsdR17(rk -, mk +) supE44 thi-1 phoA Δ(lacZYA argF)U169 Φ80lacZΔM15 λ-

DNA Alpha-Gold Efficiency
pUC19 - 2.7Kb 1.8 x 109 cfu/ug
8.2Kb plasmid 3.8 x 108 cfu/ug
13.3Kb plasmid 3.0 x 108 cfu/ug
50Kb cosmid 3.8 x 107 cfu/ug


Kit components:

  • Alpha-Gold competent cells
  • Positive Control DNA - pUC19 (10pg/μl)
  • SOC medium – 10ml

Applications:

  • lacZ marker for blue/white color screening
  • Transformation of cloned DNA into bacterial cells
  • Ideal for subcloning and generating cDNA libraries

Transformation Protocol

SKU Description Price Data sheet Delivery Shopping cart
CC100001 Alpha-Gold competent cells (20 x 50 ul) $180 pdf Next day Add to Shopping Cart
CC100002 Alpha-Gold competent cells (5 x 200 ul) $130 pdf Next day Add to Shopping Cart

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