Home Assays Fluorogenic Cell Assay Kits

Fluorogenic Cell Assay Kits

Prizm™ Fluorogenic Cell Assay Kits are based on cell-permeable fluorogenic substrates designed to monitor enzymatic activity in biological samples. These substrates diffuse through plasma or intracellular membranes by passive diffusion. Once the substrates are recognized by their corresponding protease, they are cleaved and the fragments produce fluorescence that can help measure the activity of the enzyme. Learn more…

Advantages:

Live-cell imaging: Enzymatic activity can be measured in tissues, serum and live cells without toxic effects
In vivo specificity: Fluorescent substrates assume conformations similar to active site loop conformations of naturally occurring target molecules
Measure multiple proteolytic activities: Variety of fluorophores available
Convenient: All reagents supplied in kit format, ready for immediate application
Cost-effective: Flow cytometer for quantitation or fluorometer for detection is sufficient; no modifications needed

Apoptosis Assays Cytotoxicity Assays

Apoptosis Protease Assay (Product Manual)

Prizm™ Fluorogenic Apoptosis Assay Kits are available with green λex=505 nm and Λem=530 nm ) or red (λex=552 nm and λem=580 nm ) fluorescence for the detection and measurement of caspase activities in living cells using flow cytometry or fluorescence microscopy. By combining different substrates in complementary colors, multiple caspase activities can be measured simultaneously in live cells.

SKU	Description	Price	Ship Date	Data Sheet
PL100001	Caspase-3 protease assay kit with green fluorescence substrate for 50 apoptosis assays	$550	In Stock
PL100002	Caspase-3 protease assay kit with red fluorescence substrate for 50 apoptosis assays	$550	In Stock
PL100003	Caspase-1 protease assay kit with green fluorescence substrate for 50 apoptosis assays	$550	In Stock
PL100004	Caspase-1 protease assay kit with red fluorescence substrate for 50 apoptosis assays	$550	In Stock
PL100005	Caspase-6 protease assay kit with green fluorescence substrate for 50 apoptosis assays	$550	In Stock
PL100006	Caspase-6 protease assay kit with red fluorescence substrate for 50 apoptosis assays	$550	In Stock
PL100007	Caspase-8 protease assay kit with green fluorescence substrate for 50 apoptosis assays	$550	In Stock
PL100008	Caspase-8 protease assay kit with red fluorescence substrate for 50 apoptosis assays	$550	In Stock
PL100009	Caspase-9 protease assay kit with green fluorescence substrate for 50 apoptosis assays	$550	In Stock
PL100010	Caspase-9 protease assay kit with red fluorescence substrate for 50 apoptosis assays	$550	In Stock

Prizm™ Cell-Mediated Cytotoxicity Assay (Product Manual)

Prizm^TM Fluorogenic Cell-Mediated Cytotoxicity Assay are available with green fluorescence for the detection and measurement of Caspase and Granzyme B activities following the successful delivery of a lethal hit by cytotoxic lymphocytes. Enzyme activity can be measured either by flow cytometry or fluorescence microscopy.

SKU	Description	Price	Ship Date	Data Sheet
PL100011	Caspase-6 protease substrate kit for 80 cell-mediated cytotoxicity assays	$600	In Stock
PL100012	Granzyme-B protease substrate kit for 80 cell-mediated cytotoxicity assays	$600	In Stock
PL100013	Granzyme B & Caspase-8 proteases substrate kit for 80 cell-mediated cytotoxicity assays	$600	In Stock

Assays

Luminex Antibody Pairs

ELISA Antibody Pairs

Luminex Kits

Autoantibody Profiling Array