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Fluorogenic Cell Assay Kits

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Cell_Assay.gifPrizm™ Fluorogenic Cell Assay Kits are based on cell-permeable fluorogenic substrates designed to monitor enzymatic activity in biological samples. These substrates diffuse through plasma or intracellular membranes by passive diffusion. Once the substrates are recognized by their corresponding protease, they are cleaved and the fragments produce fluorescence that can help measure the activity of the enzyme. Learn more…

Advantages:

  • Live-cell imaging: Enzymatic activity can be measured in tissues, serum and live cells without toxic effects
  • In vivo specificity: Fluorescent substrates assume conformations similar to active site loop conformations of naturally occurring target molecules
  • Measure multiple proteolytic activities: Variety of fluorophores available
  • Convenient: All reagents supplied in kit format, ready for immediate application
  • Cost-effective: Flow cytometer for quantitation or fluorometer for detection is sufficient; no modifications needed

Apoptosis Assays     Cytotoxicity Assays

Apoptosis Protease Assay (Product Manual)

Prizm™ Fluorogenic Apoptosis Assay Kits are available with green λex=505 nm and Λem=530 nm ) or red (λex=552 nm and λem=580 nm ) fluorescence for the detection and measurement of caspase activities in living cells using flow cytometry or fluorescence microscopy. By combining different substrates in complementary colors, multiple caspase activities can be measured simultaneously in live cells.

SKU Description Price Ship Date Data Sheet
PL100001 Caspase-3 protease assay kit with green fluorescence substrate for 50 apoptosis assays $550 In Stock pdf Add to Shopping Cart
PL100002 Caspase-3 protease assay kit with red fluorescence substrate for 50 apoptosis assays $550 In Stock pdf Add to Shopping Cart
PL100003 Caspase-1 protease assay kit with green fluorescence substrate for 50 apoptosis assays $550 In Stock pdf Add to Shopping Cart
PL100004 Caspase-1 protease assay kit with red fluorescence substrate for 50 apoptosis assays $550 In Stock pdf Add to Shopping Cart
PL100005 Caspase-6 protease assay kit with green fluorescence substrate for 50 apoptosis assays $550 In Stock pdf Add to Shopping Cart
PL100006 Caspase-6 protease assay kit with red fluorescence substrate for 50 apoptosis assays $550 In Stock pdf Add to Shopping Cart
PL100007 Caspase-8 protease assay kit with green fluorescence substrate for 50 apoptosis assays $550 In Stock pdf Add to Shopping Cart
PL100008 Caspase-8 protease assay kit with red fluorescence substrate for 50 apoptosis assays $550 In Stock pdf Add to Shopping Cart
PL100009 Caspase-9 protease assay kit with green fluorescence substrate for 50 apoptosis assays $550 In Stock pdf Add to Shopping Cart
PL100010 Caspase-9 protease assay kit with red fluorescence substrate for 50 apoptosis assays $550 In Stock pdf Add to Shopping Cart

Prizm™ Cell-Mediated Cytotoxicity Assay (Product Manual)

PrizmTM Fluorogenic Cell-Mediated Cytotoxicity Assay are available with green fluorescence for the detection and measurement of Caspase and Granzyme B activities following the successful delivery of a lethal hit by cytotoxic lymphocytes. Enzyme activity can be measured either by flow cytometry or fluorescence microscopy.

SKU Description Price Ship Date Data Sheet
PL100011 Caspase-6 protease substrate kit for 80 cell-mediated cytotoxicity assays $600 In Stock pdf Add to Shopping Cart
PL100012 Granzyme-B protease substrate kit for 80 cell-mediated cytotoxicity assays $600 In Stock pdf Add to Shopping Cart
PL100013 Granzyme B & Caspase-8 proteases substrate kit for 80 cell-mediated cytotoxicity assays $600 In Stock pdf Add to Shopping Cart