Register | Shopping Cart | Blast your Gene | Home

OriGene cDNA clones in recent publications

Expression and Function of Epithelial Anoctamins J. Biol. Chem., Mar 2010; 285: 7838 - 7845 [ANO10]

Retinoid-suppressed phosphorylation of RARa mediates the differentiation pathway of osteosarcoma cells Oncogene (1 March 2010) doi:10.1038/onc.2010.50 Original Article [RARA]

A chemical and phosphoproteomic characterization of dasatinib action in lung cancer Nature Chemical Biology (28 February 2010) doi:10.1038/nchembio.332 Article [PTK6]

A Comparison of the Roles of Peroxisome Proliferator-Activated Receptor and Retinoic Acid Receptor on CYP26 Regulation Mol. Pharmacol., Feb 2010; 77: 218 - 227 [CYP26B1]

View All Citations >>

VCL (NM_003373) Human cDNA ORF Clone

Western Blot Validated

Specifications

Related Products

Product Manual

FAQs

Cat. No.

Ref. ID

Description

Vector

Price

Delivery

RC204576

NM_003373

Myc-DDK-tagged ORF clone of Homo sapiens vinculin (VCL), transcript variant 2 as transfection-ready DNA

$680

Immediate

Click here for subcloning services of this insert
into destination vectors

Please select amount:

Also for VCL (NM_003373)

Protein Request

Antibody Search

OriGene TrueORF Data

Vector:	pCMV6 Entry	Expression Tag: C-terminal MYC/DDK	Western validation with an anti-DDK antibody L: Control HEK293 lysate R: Over-expression lysate
Sequence Data:	ORF Nucleotide Sequence Protein Sequence Chromatogram	ORF Size: 3201 bp Predicted Protein MW: 116.5 kDa
Restriction Sites:	Cloning Scheme for this gene
OTI Annotation:	This clone expresses the complete ORF with an expression tag.
OTI Disclaimer:	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components:	The ORF clone is shipped in a 2-D bar-coded Matrix tube as 10 ug transfection-ready dried plasmid DNA.

Reference Data

RefSeq:NM_003373.3, NP_003364	RefSeq Size: 5443	RefSeq ORF: 3201
Synonyms : CMD1W; MVCL
LocusID: 7414	Cytogenetic: 10q22.2	Domains: Vinculin
Summary: Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq].

OriGene offers custom subcloning services to shuttle this insert into any of our destination vectors via PrecisionShuttle for an additional $400 service charge. The delivery takes additional 2 weeks.$1080

Epitope tagging

FP tagging

Selection markers

Inducible

Bacterial expression

Destination Vector	C-Tag	N-Tag	E. coli Selection	Cell Selection	Expression	Custom Subcloning	Delivery
pCMV6-AC-His	His		Ampicillin	Neomycin	Mammalian	$1080	2 weeks
pCMV6-AC-Myc	Myc		Ampicillin	Neomycin	Mammalian	$1080	2 weeks
pCMV6-AC-HA	HA		Ampicillin	Neomycin	Mammalian	$1080	2 weeks
pCMV6-AC-DDK	DDK		Ampicillin	Neomycin	Mammalian	$1080	2 weeks
pCMV6-AC-Myc-His	Myc-His		Ampicillin	Neomycin	Mammalian	$1080	2 weeks
pCMV6-AC-Myc-DDK	Myc-DDK		Ampicillin	Neomycin	Mammalian	$1080	2 weeks
pCMV6-AC-HA-His	HA-His		Ampicillin	Neomycin	Mammalian	$1080	2 weeks
pCMV6-AC-DDK-His	DDK-His		Ampicillin	Neomycin	Mammalian	$1080	2 weeks
pCMV6-AN-His		His	Ampicillin	Neomycin	Mammalian	$1080	2 weeks
pCMV6-AN-Myc		Myc	Ampicillin	Neomycin	Mammalian	$1080	2 weeks
pCMV6-AN-HA		HA	Ampicillin	Neomycin	Mammalian	$1080	2 weeks
pCMV6-AN-DDK		DDK	Ampicillin	Neomycin	Mammalian	$1080	2 weeks
pCMV6-AN-His-Myc		His-Myc	Ampicillin	Neomycin	Mammalian	$1080	2 weeks
pCMV6-AN-Myc-DDK		Myc-DDK	Ampicillin	Neomycin	Mammalian	$1080	2 weeks
pCMV6-AN-His-HA		His-HA	Ampicillin	Neomycin	Mammalian	$1080	2 weeks
pCMV6-AN-His-DDK		His-DDK	Ampicillin	Neomycin	Mammalian	$1080	2 weeks

Peptide sequence of the DDK-tag (Flag®): N-DYKDDDDK-C
Flag® is a registered trademark of Sigma-Aldrich

Copyright © 2010 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.