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Home cDNA Clone TrueORF All MCM4 ORF Clones
TrueORF Gold

MCM4 (NM_182746) Human cDNA ORF Clone

Specifications Citations Clones of Other Species Product Documents
Cat. No. Description Price Availability  
RC206122 MCM4 (Myc-DDK-tagged)-Human minichromosome maintenance complex component 4 (MCM4), transcript variant 2, 10µg
$540
In Stock
RC506122 MCM4 (Myc-DDK-tagged)-Human minichromosome maintenance complex component 4 (MCM4), transcript variant 2, (200ug) $660 2~3 weeks
TA50011-100 4C5, Anti-DDK monoclonal antibody, 100µl $248 In Stock
Clone Modification

The same insert cloned in Lenti and other vectors is also available.

Cat. No. Description Vector Price Availability  
RC206122L2 Lenti ORF clone of Human minichromosome maintenance complex component 4 (MCM4), transcript variant 2, mGFP tagged

1000 3 Weeks
TR30022 Lenti-vpak packaging kit - packaging plasmids and transfection reagent (10 rxns) Lenti-vpak-app-guide $390 In Stock
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Also for MCM4 (NM_182746)
cDNA Clone shRNA/siRNA Lysate Protein Antibody
TrueORF Data for RC206122
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Vector: pCMV6-Entry   Change vector? Tag: C-terminal Myc-DDK
Western validation with an anti-DDK antibody *
L: Control HEK293 lysate
R: Over-expression lysate
Lysate_Image
Sequence Data: ORF Nucleotide Sequence
Protein Sequence
ORF Size: 2592 bp
Predicted Protein MW: 96.4 kDa
Restriction Sites: SgfI-MluI     Cloning Scheme for this gene     Plasmid Map Plasmid Map
OTI Annotation: This clone was engineered to express the complete ORF with an expression tag.
OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Protein Families: Stem cell - PluripotencyTranscription Factors
Protein Pathways: DNA replicationCell cycle
* The lysates used for this WB picture contain the overexpressed empty vector or the Myc-DDK tagged ORF clone.

Reference Data
RefSeq: NM_182746.1, NP_877423
RefSeq Size: 3417 RefSeq ORF: 2592
Synonyms : CDC21; CDC54; hCdc21; NKCD; NKGCD; P1-CDC21
LocusID: 4173 Cytogenetic: 8q11.2
Summary: The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008].

 

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