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Home cDNA Clone TrueORF All PKM ORF Clones
TrueORF Gold

PKM (NM_182470) Human cDNA ORF Clone

Specifications Citations (1) Clones of Other Species Product Documents
Cat. No. Description Price Availability  
RC219382 PKM (Myc-DDK-tagged)-Human pyruvate kinase, muscle (PKM2), transcript variant 2, 10µg   
$660
In Stock
RG219382 PKM (GFP-tagged) - Human pyruvate kinase, muscle (PKM2), transcript variant 2, (10ug) $720 In Stock
TA50011-100 4C5, Anti-DDK monoclonal antibody, 100µl $248 In Stock
Cat. No. Description Price Availability
RC519382 PKM (Myc-DDK-tagged)-Human pyruvate kinase, muscle (PKM2), transcript variant 2, (200ug) $780 2 weeks
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The same insert cloned in a GFP-tagging vector is also available

Cat. No. Description Vector Price Availability  
RG219382 PKM (GFP-tagged) - Human pyruvate kinase, muscle (PKM2), transcript variant 2, (10ug)

$720 In Stock
TA150041 2H8, Anti-tGFP monoclonal antibody, 100µl Datasheet $248 In Stock
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TrueORF Data for RC219382
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Vector: pCMV6-Entry   Change vector? Tag: C-terminal Myc-DDK
Western validation with an anti-DDK antibody *
L: Control HEK293 lysate
R: Over-expression lysate
Lysate_Image
Sequence Data: ORF Nucleotide Sequence
Protein Sequence
ORF Size: 1596 bp
Predicted Protein MW: 57.9 kDa
Restriction Sites: SgfI-MluI     Cloning Scheme for this gene     Plasmid Map Plasmid Map
OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Protein Families: Druggable Genome
Protein Pathways: Glycolysis / GluconeogenesisPurine metabolismPyruvate metabolismMetabolic pathwaysType II diabetes mellitus
* The lysates used for this WB picture contain the overexpressed empty vector or the Myc-DDK tagged ORF clone.

Reference Data
RefSeq Explanation: NM_182470.1, NP_872270 RefSeq Size: 2674 RefSeq ORF: 1596
Synonyms : CTHBP; HEL-S-30; OIP3; PK3; PKM2; TCB; THBP1
LocusID: 5315 Cytogenetic: 15q22
Gene Summary: This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. [provided by RefSeq, May 2011].

 

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