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Home cDNA Clone TrueORF All CDC25B ORF Clones
TrueORF Gold

CDC25B (NM_021873) Human cDNA ORF Clone

Specifications Citations Clones of Other Species Product Documents
Cat. No. Description Price Availability  
RC207409 CDC25B (Myc-DDK-tagged)-Human cell division cycle 25 homolog B (S. pombe) (CDC25B), transcript variant 1, 10µg   
$560
In Stock
RG207409 CDC25B (GFP-tagged) - Human cell division cycle 25 homolog B (S. pombe) (CDC25B), transcript variant 1, (10ug) $610 In Stock
TA50011-100 4C5, Anti-DDK monoclonal antibody, 100µl $248 In Stock
Cat. No. Description Price Availability
RC507409 CDC25B (Myc-DDK-tagged)-Human cell division cycle 25 homolog B (S. pombe) (CDC25B), transcript variant 1, (200ug) $680 2 weeks
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Clone Modification
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The same insert cloned in a GFP-tagging vector is also available

Cat. No. Description Vector Price Availability  
RG207409 CDC25B (GFP-tagged) - Human cell division cycle 25 homolog B (S. pombe) (CDC25B), transcript variant 1, (10ug)

$610 In Stock
TA150041 2H8, Anti-tGFP monoclonal antibody, 100µl Datasheet $248 In Stock
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TrueORF Data for RC207409
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Vector: pCMV6-Entry   Change vector? Tag: C-terminal Myc-DDK
Western validation with an anti-DDK antibody *
L: Control HEK293 lysate
R: Over-expression lysate
Lysate_Image
Sequence Data: ORF Nucleotide Sequence
Protein Sequence
ORF Size: 1743 bp
Predicted Protein MW: 64.8 kDa
Restriction Sites: SgfI-MluI     Cloning Scheme for this gene     Plasmid Map Plasmid Map
OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Protein Families: PhosphataseDruggable Genome
Protein Pathways: MAPK signaling pathwayCell cycleProgesterone-mediated oocyte maturation
* The lysates used for this WB picture contain the overexpressed empty vector or the Myc-DDK tagged ORF clone.

Reference Data
RefSeq Explanation: NM_021873.2, NP_068659 RefSeq Size: 3701 RefSeq ORF: 1743
Synonyms : OTTHUMP00000030137; OTTHUMP00000030138; OTTHUMP00000030139; cell division cycle 25B; cell division cycle 25 homolog B (S. pombe)
LocusID: 994 Cytogenetic: 20p13 Domains: RHOD
Gene Summary: CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist. [provided by RefSeq, Jul 2008].

 

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