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Home cDNA Clone TrueORF All MGST1 ORF Clones
TrueORF Gold

MGST1 (NM_020300) Human cDNA ORF Clone

Specifications Citations Clones of Other Species Product Documents
Cat. No. Description Price Availability  
RC203251 MGST1 (Myc-DDK-tagged)-Human microsomal glutathione S-transferase 1 (MGST1), transcript variant 1b, 10µg
$380
In Stock
RC503251 MGST1 (Myc-DDK-tagged)-Human microsomal glutathione S-transferase 1 (MGST1), transcript variant 1b, (200ug) $500 2~3 weeks
TA50011-100 4C5, Anti-DDK monoclonal antibody, 100µl $248 In Stock
Clone Modification

The same insert cloned in Lenti and other vectors is also available.

Cat. No. Description Vector Price Availability  
RC203251L2 Lenti ORF clone of Human microsomal glutathione S-transferase 1 (MGST1), transcript variant 1b, mGFP tagged

830 3 Weeks
TR30022 Lenti-vpak packaging kit - packaging plasmids and transfection reagent (10 rxns) Lenti-vpak-app-guide $390 In Stock
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TrueORF Data for RC203251
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Vector: pCMV6-Entry   Change vector? Tag: C-terminal Myc-DDK
Western validation with an anti-DDK antibody *
L: Control HEK293 lysate
R: Over-expression lysate
Lysate_Image
Sequence Data: ORF Nucleotide Sequence
Protein Sequence
ORF Size: 468 bp
Predicted Protein MW: 17.4 kDa
Restriction Sites: SgfI-MluI     Cloning Scheme for this gene     Plasmid Map Plasmid Map
OTI Annotation: This clone was engineered to express the complete ORF with an expression tag.
OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Protein Families: TransmembraneDruggable Genome
Protein Pathways: Glutathione metabolismMetabolism of xenobiotics by cytochrome P450Drug metabolism - cytochrome P450
* The lysates used for this WB picture contain the overexpressed empty vector or the Myc-DDK tagged ORF clone.

Reference Data
RefSeq: NM_020300.3, NP_064696
RefSeq Size: 953 RefSeq ORF: 468
Synonyms : GST12; MGST; MGST-I
LocusID: 4257 Cytogenetic: 12p12.3-p12.1 Domains: MAPEG
Summary: The MAPEG (Membrane Associated Proteins in Eicosanoid and Glutathione metabolism) family consists of six human proteins, two of which are involved in the production of leukotrienes and prostaglandin E, important mediators of inflammation. Other family members, demonstrating glutathione S-transferase and peroxidase activities, are involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds. This gene encodes a protein that catalyzes the conjugation of glutathione to electrophiles and the reduction of lipid hydroperoxides. This protein is localized to the endoplasmic reticulum and outer mitochondrial membrane where it is thought to protect these membranes from oxidative stress. Several transcript variants, some non-protein coding and some protein coding, have been found for this gene. [provided by RefSeq, May 2012].

 

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