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Home cDNA Clone TrueORF All AP2A1 ORF Clones

AP2A1 (NM_014203) Human cDNA ORF Clone

Western Blot Validated

Specifications Citations Clones of Other Species Product Documents
Cat. No. Description Price Availability  
RC224855 AP2A1 (Myc-DDK-tagged)-Human adaptor-related protein complex 2, alpha 1 subunit (AP2A1), transcript variant 1, 10µg
$1410
In Stock
TA50011-100 4C5, Anti-DDK monoclonal antibody, 100µl $248 In Stock
Clone Modification

The same insert cloned in Lenti and other vectors is also available.

Cat. No. Description Vector Price Availability  
RC224855L2 Lenti-ORF clone of AP2A1 (mGFP-tagged)-Human adaptor-related protein complex 2, alpha 1 subunit (AP2A1), transcript variant 1, (10ug)

1960
$1710
3 Weeks
TR30022 Lenti-vpak packaging kit - packaging plasmids and transfection reagent (10 rxns) Lenti-vpak-app-guide $390 In Stock
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TrueORF Data for RC224855
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Vector: pCMV6-Entry   Change vector? Tag: C-terminal Myc-DDK
Western validation with an anti-DDK antibody *
L: Control HEK293 lysate
R: Over-expression lysate
Lysate_Image
Sequence Data: ORF Nucleotide Sequence
Protein Sequence
ORF Size: 2934 bp
Predicted Protein MW: 107.4 kDa
Restriction Sites: SgfI-MluI     Cloning Scheme for this gene     Plasmid Map Plasmid Map
OTI Annotation: This clone was engineered to express the complete ORF with an expression tag.
OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Protein Pathways: EndocytosisHuntington's disease
* The lysates used for this WB picture contain the overexpressed empty vector or the Myc-DDK tagged ORF clone.

Reference Data
RefSeq: NM_014203.2, NP_055018
RefSeq Size: 3499 RefSeq ORF: 2934
Synonyms : ADTAA; AP2-ALPHA; CLAPA1
LocusID: 160 Cytogenetic: 19q13.33 Domains: Adaptin_N, Alpha_adaptin_C, Alpha_adaptinC2
Summary: This gene encodes the alpha 1 adaptin subunit of the adaptor protein 2 (AP-2) complex found in clathrin coated vesicles. The AP-2 complex is a heterotetramer consisting of two large adaptins (alpha or beta), a medium adaptin (mu), and a small adaptin (sigma). The complex is part of the protein coat on the cytoplasmic face of coated vesicles which links clathrin to receptors in vesicles. Alternative splicing of this gene results in two transcript variants encoding two different isoforms. A third transcript variant has been described, but its full length nature has not been determined. [provided by RefSeq, Jul 2008].

 

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