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Home cDNA Clone TrueORF All POLR2J ORF Clones
TrueORF Gold

POLR2J (NM_006234) Human cDNA ORF Clone

Specifications Citations Clones of Other Species Product Documents
Cat. No. Description Price Availability  
RC204819 POLR2J (Myc-DDK-tagged)-Human polymerase (RNA) II (DNA directed) polypeptide J, 13.3kDa (POLR2J), 10µg
$380
In Stock
RC504819 POLR2J (Myc-DDK-tagged)-Human polymerase (RNA) II (DNA directed) polypeptide J, 13.3kDa (POLR2J), (200ug) $500 2~3 weeks
TA50011-100 4C5, Anti-DDK monoclonal antibody, 100µl $248 In Stock
Clone Modification

The same insert cloned in Lenti and other vectors is also available.

Cat. No. Description Vector Price Availability  
RC204819L2 Lenti ORF clone of Human polymerase (RNA) II (DNA directed) polypeptide J, 13.3kDa (POLR2J), mGFP tagged

$830 3 Weeks
TR30022 Lenti-vpak packaging kit - packaging plasmids and transfection reagent (10 rxns) Lenti-vpak-app-guide $390 In Stock
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Also for POLR2J (NM_006234)
cDNA Clone shRNA/siRNA Lysate Protein Antibody
TrueORF Data for RC204819
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Vector: pCMV6-Entry   Change vector? Tag: C-terminal Myc-DDK
Western validation with an anti-DDK antibody *
L: Control HEK293 lysate
R: Over-expression lysate
Lysate_Image
Sequence Data: ORF Nucleotide Sequence
Protein Sequence
ORF Size: 354 bp
Predicted Protein MW: 13.1 kDa
Restriction Sites: SgfI-MluI     Cloning Scheme for this gene     Plasmid Map Plasmid Map
OTI Annotation: This clone was engineered to express the complete ORF with an expression tag.
OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Protein Families: Transcription Factors
Protein Pathways: Purine metabolismPyrimidine metabolismMetabolic pathwaysRNA polymeraseHuntington's disease
* The lysates used for this WB picture contain the overexpressed empty vector or the Myc-DDK tagged ORF clone.

Reference Data
RefSeq: NM_006234.4, NP_006225
RefSeq Size: 991 RefSeq ORF: 354
Synonyms : hRPB14; POLR2J1; RPB11; RPB11A; RPB11m
LocusID: 5439 Cytogenetic: 7q22.1 Domains: RNA_pol_L
Summary: This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene exists as a heterodimer with another polymerase subunit; together they form a core subassembly unit of the polymerase. Two similar genes are located nearby on chromosome 7q22.1 and a pseudogene is found on chromosome 7p13. [provided by RefSeq, Jul 2008].

 

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