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Home cDNA Clone TrueORF All NEFL ORF Clones
TrueORF Gold

NEFL (NM_006158) Human cDNA ORF Clone

Specifications Citations Clones of Other Species Product Documents
Cat. No. Description Price Availability  
RC205920 NEFL (Myc-DDK-tagged)-Human neurofilament, light polypeptide (NEFL), 10µg
$540
In Stock
RC505920 NEFL (Myc-DDK-tagged)-Human neurofilament, light polypeptide (NEFL), (200ug) $660 2~3 weeks
TA50011-100 4C5, Anti-DDK monoclonal antibody, 100µl $248 In Stock
Clone Modification

The same insert cloned in Lenti and other vectors is also available.

Cat. No. Description Vector Price Availability  
RC205920L2 Lenti ORF clone of Human neurofilament, light polypeptide (NEFL), mGFP tagged

$1000 2 Weeks
TR30022 Lenti-vpak packaging kit - packaging plasmids and transfection reagent (10 rxns) Lenti-vpak-app-guide $390 In Stock
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Also for NEFL (NM_006158)
cDNA Clone shRNA/siRNA Lysate Protein Antibody
TrueORF Data for RC205920
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Vector: pCMV6-Entry   Change vector? Tag: C-terminal Myc-DDK
Western validation with an anti-DDK antibody *
L: Control HEK293 lysate
R: Over-expression lysate
Lysate_Image
Sequence Data: ORF Nucleotide Sequence
Protein Sequence
ORF Size: 1632 bp
Predicted Protein MW: 61.3 kDa
Restriction Sites: SgfI-MluI     Cloning Scheme for this gene     Plasmid Map Plasmid Map
OTI Annotation: This clone was engineered to express the complete ORF with an expression tag.
OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Protein Families: ES Cell Differentiation/IPSDruggable Genome
Protein Pathways: Amyotrophic lateral sclerosis (ALS)
* The lysates used for this WB picture contain the overexpressed empty vector or the Myc-DDK tagged ORF clone.

Reference Data
RefSeq: NM_006158.2, NP_006149
RefSeq Size: 3584 RefSeq ORF: 1632
Synonyms : CMT1F; CMT2E; NF-L; NF68; NFL
LocusID: 4747 Cytogenetic: 8p21 Domains: filament, filament_head
Summary: Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq, Oct 2008].

 

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