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Home cDNA Clone TrueORF All SAE1 ORF Clones
TrueORF Gold

SAE1 (NM_005500) Human cDNA ORF Clone

Specifications Citations (1) Clones of Other Species Product Documents
Cat. No. Description Price Availability  
RC201820 SAE1 (Myc-DDK-tagged)-Human SUMO1 activating enzyme subunit 1 (SAE1), transcript variant 1, 10µg   
$560
In Stock
RG201820 SAE1 (GFP-tagged) - Human SUMO1 activating enzyme subunit 1 (SAE1), transcript variant 1, (10ug) $610 In Stock
TA50011-100 4C5, Anti-DDK monoclonal antibody, 100µl $248 In Stock
Cat. No. Description Price Availability
RC501820 SAE1 (Myc-DDK-tagged)-Human SUMO1 activating enzyme subunit 1 (SAE1), transcript variant 1, (200ug) $680 2 weeks
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The same insert cloned in a GFP-tagging vector is also available

Cat. No. Description Vector Price Availability  
RG201820 SAE1 (GFP-tagged) - Human SUMO1 activating enzyme subunit 1 (SAE1), transcript variant 1, (10ug)

$610 In Stock
TA150041 2H8, Anti-tGFP monoclonal antibody, 100µl Datasheet $248 In Stock
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TrueORF Data for RC201820
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Vector: pCMV6-Entry   Change vector? Tag: C-terminal Myc-DDK
Western validation with an anti-DDK antibody *
L: Control HEK293 lysate
R: Over-expression lysate
Lysate_Image
Sequence Data: ORF Nucleotide Sequence
Protein Sequence
ORF Size: 1041 bp
Predicted Protein MW: 38.3 kDa
Restriction Sites: SgfI-MluI     Cloning Scheme for this gene     Plasmid Map Plasmid Map
OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Protein Pathways: Ubiquitin mediated proteolysis
* The lysates used for this WB picture contain the overexpressed empty vector or the Myc-DDK tagged ORF clone.

Reference Data
RefSeq Explanation: NM_005500.1, NP_005491 RefSeq Size: 2119 RefSeq ORF: 1041
Synonyms : AOS1; HSPC140; SUA1; UBLE1A
LocusID: 10055 Cytogenetic: 19q13.32 Domains: ThiF
Gene Summary: Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 (MIM 613295) form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM, Mar 2010].

 

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