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 Home cDNA Clone TrueORF All BRAF ORF Clones
TrueORF Gold

BRAF (NM_004333) Human cDNA ORF Clone

Specifications Citations (1) Clone of Other Species Product Documents
Cat. No. Description Price Availability  
RC211013 Myc-DDK-tagged ORF clone of Homo sapiens v-raf murine sarcoma viral oncogene homolog B1 (BRAF) as transfection-ready DNA, 10µg
$540
 In Stock
TA50011-100 4C5, Anti-DDK monoclonal antibody, 100µl $248
$198		 In Stock

$50 off anti-DDK and other anti-tag antibodies till June 30th, 2013

The same insert cloned in Lenti and other vectors is also available.

Cat. No. Description Vector Price Availability  
RC211013L1 Lenti ORF clone of Human v-raf murine sarcoma viral oncogene homolog B1 (BRAF) , Myc-DDK-tagged

$910 2 Weeks
TR30022 Lenti-vpak packaging kit - packaging plasmids and transfection reagent (10 rxns) Lenti-vpak-app-guide $360 In Stock
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Also for BRAF (NM_004333)
cDNA Clone shRNA/siRNA Lysate Protein Antibody
TrueORF Data for RC211013
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Vector: pCMV6-Entry   Change vector? Tag: C-terminal Myc-DDK
Western validation with an anti-DDK antibody *
L: Control HEK293 lysate
R: Over-expression lysate
Lysate_Image
Sequence Data: ORF Nucleotide Sequence
Protein Sequence 		ORF Size: 2301 bp
Predicted Protein MW: 84.3 kDa
Restriction Sites: SgfI-MluI     Cloning Scheme for this gene     Plasmid Map Plasmid Map
OTI Annotation: This clone was engineered to express the complete ORF with an expression tag.
OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Protein Families: Protein KinaseDruggable Genome
Protein Pathways: MAPK signaling pathwayErbB signaling pathwayChemokine signaling pathwaymTOR signaling pathwayVascular smooth muscle contractionFocal adhesionMore Pathways >>
* The lysates used for this WB picture contain the overexpressed empty vector or the Myc-DDK tagged ORF clone.

Reference Data
RefSeq: NM_004333.3, NP_004324
 RefSeq Size: 2477 RefSeq ORF: 2301
Synonyms : B-RAF1; BRAF1; NS7; RAFB1
LocusID: 673 Cytogenetic: 7q34 Domains: pkinase, TyrKc, C1, S_TKc, RBD
Summary: This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq, Jul 2008].

 
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